Inhibitory effects of chitooligosaccharides on degranulation and cytokine generation in rat basophilic leukemia RBL-2H3 cells

Chitooligosaccharides (COS) of three different molecular weight ranges (1–3kDa, 3–5kDa and 5–10kDa) were investigated for their abilities against allergic reactions in RBL-2H3 cells. At the high concentration of 1000μg/ml, COS with molecular weights of 1–3kDa greatly attenuated the histamine and β-h...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate polymers 2011-02, Vol.84 (1), p.649-655
Main Authors: Vo, Thanh-Sang, Kong, Chang-Suk, Kim, Se-Kwon
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
calcium
Calcium ionophore A23187
Chitooligosaccharides
Cytokine
Exact sciences and technology
gene expression
Histamine
Histamine and antagonists. Allergy
inflammation
interleukin-4
interleukin-6
Intracellular Ca2
leukemia
Medical sciences
messenger RNA
mitogen-activated protein kinase
molecular weight
Natural polymers
Pharmacology. Drug treatments
phosphorylation
Physicochemistry of polymers
rats
RBL-2H3 cells
Starch and polysaccharides
tumor necrosis factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chitooligosaccharides (COS) of three different molecular weight ranges (1–3kDa, 3–5kDa and 5–10kDa) were investigated for their abilities against allergic reactions in RBL-2H3 cells. At the high concentration of 1000μg/ml, COS with molecular weights of 1–3kDa greatly attenuated the histamine and β-hexosaminidase release and intracellular Ca2+ elevation induced by calcium ionophore A23187, at the rates of 32% and 34% release and 37% elevation, respectively. Furthermore, the inhibitory activities of COS on calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA)-induced production as well as mRNA expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4, and IL-6 were evidenced. Notably, the suppressive effects on the phosphorylation of extracellular signal-regulated kinases (MEK/ERK) and p38 kinase by COS were confirmed. These results indicate that COS could be used as an inhibitor in regulating mast cell-mediated allergic inflammatory responses.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2010.12.046