An anticoagulant effect and chemical characterization of Lythrum salicaria L. glycoconjugates

► Water extract of Lythrum salicaria is resolved into twelve glycoconjugates according to the negative charge. ► Individual glycoconjugates differ in carbohydrate, protein and phenolic contents. ► Three glycoconjugate fractions possess higher anticoagulant activity as that of the native water extrac...

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Bibliographic Details
Published in:Carbohydrate polymers 2011-08, Vol.86 (1), p.277-284
Main Authors: Pawlaczyk, Izabela, Capek, Peter, Czerchawski, Leszek, Bijak, Joanna, Lewik-Tsirigotis, Marta, Pliszczak-Król, Aleksandra, Gancarz, Roman
Format: Article
Language:English
Subjects:
Anticoagulant activity
Applied sciences
arabinogalactans
Biological and medical sciences
Blood. Blood coagulation. Reticuloendothelial system
Chemical constitution
Exact sciences and technology
FT-IR
Fundamental and applied biological sciences. Psychology
Glycoconjugates
ion exchange
ion exchange chromatography
Lythrum salicaria
Lythrum salicaria L
Medical sciences
Medicinal plant
molecular weight
Natural polymers
NMR
Pharmacology. Drug treatments
Physicochemistry of polymers
Plant physiology and development
Procoagulant activity
sodium chloride
sodium hydroxide
Starch and polysaccharides
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Summary:► Water extract of Lythrum salicaria is resolved into twelve glycoconjugates according to the negative charge. ► Individual glycoconjugates differ in carbohydrate, protein and phenolic contents. ► Three glycoconjugate fractions possess higher anticoagulant activity as that of the native water extract. ► The anticoagulant activity increases with uronic acids and phenolics contents. An ion-exchange chromatography of Lythrum salicaria glycoconjugate afforded twelve fractions (LsF1–F12) by stepwise elution with water, sodium chloride and sodium hydroxide solutions. Carbohydrate, phenolic and protein moieties have been determined in all fractions. Individual glycoconjugates LsF1–F12 varied in carbohydrate, phenolic and protein contents, molecular mass and monosaccharide composition. Rhamnogalacturonans and/or homogalacturonans associated with arabinogalactans, arabinan and hexosan types of polysaccharides were identified in ion-exchange fractions. In vitro anticoagulant activity tests showed complete inhibition of plasma clot formation by LsF2, LsF7 and LsF12 fractions and a slight procoagulant effect of LsF3 and LsF4 ones while other ion-exchange fractions were not active.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2011.04.048