A transcriptomic approach to predict the impact of β-(1,3)-polyglucuronic acid sodium salt and derivatives in the main biological processes

• Published in: Carbohydrate polymers 2012-01, Vol.87 (2), p.1828-1836
• Main Authors: Delattre, C., Michaud, P., Chaisemartin, L., Berthon, J.Y., Rios, L.
• Format: Article
• Language: English
• Subjects: Applied sciences; Biological processes; Curdlan; DNA; DNA-array; Exact sciences and technology; fibroblasts; genes; humans; Natural polymers; oxidation; Physicochemistry of polymers; Regioselective oxidation; sodium hypochlorite; Starch and polysaccharides; Transcriptomic analysis; transcriptomics; β-(1,3)-Polyglucuronic acid sodium salt
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2011.10.010

► A new β-(1,3)-polyglucuronic acid sodium salt from regioselective oxidation of curdlan. ► Radical and thermal depolymerisations of β-(1,3)-polyglucuronic acid sodium salt. ► Screening of the main biological processes implicated by β-(1,3)-polyglucuronic acid sodium salt and sulphated/acetylated derivatives. ► A transcriptomic method to screen the impact of β-(1,3)-polyglucuronic acid sodium salt and derivatives in biological process. β-(1,3)-Polyglucuronic acid sodium salt was produced by the regioselective oxidation of curdlan using 2,2,6,6,-tetramethylpiperidine-1-oxyl radical (TEMPO)/NaBr/NaClO systems. This β-(1,3)-polyuronic acid sodium salt was depolymerized, O-sulphated and O-acetylated. All these molecules have been biologically screened for transcriptomic analysis using DNA array. The transcriptomical responses were studied after statistical gene ontology in order to evaluate biological responses disturbed by the individual treatments of human fibroblast by all these β-(1,3)-polyglucuronic acid sodium salt derivatives.