Jellyfish skin polysaccharides: Extraction and inhibitory activity on macrophage-derived foam cell formation

In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP). The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h. Under these condition...

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Bibliographic Details
Published in:Carbohydrate polymers 2014-06, Vol.106, p.393-402
Main Authors: ZHANG, Hai-Lin, CUI, Shao-Hua, ZHA, Xue-Qiang, BANSAL, Vibha, LEI XUE, LI, Xiao-Long, RAN HAO, PAN, Li-Hua, LUO, Jian-Ping
Format: Article
Language:English
Subjects:
1-Butanol - pharmacology
Animals
Applied sciences
Atherosclerosis - genetics
Biological and medical sciences
Cardiovascular system
Cell Line
Chemical Precipitation
Chloroform - pharmacology
Cholesterol - analysis
Down-Regulation - drug effects
Drug Evaluation, Preclinical
Ethanol - pharmacology
Exact sciences and technology
Foam Cells - chemistry
Foam Cells - drug effects
Gene Expression Regulation - drug effects
Lipoproteins, LDL - pharmacology
Macrophages - chemistry
Macrophages - drug effects
Medical sciences
Mice
Molecular Weight
Natural polymers
Papain - pharmacology
Pharmacology. Drug treatments
Physicochemistry of polymers
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Real-Time Polymerase Chain Reaction
Scyphozoa - chemistry
Solvents - pharmacology
Spectrophotometry, Infrared
Starch and polysaccharides
Vascular wall
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Summary:In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP). The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h. Under these conditions, the JSP yield reached 1.007 mg/g. Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP. After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively. The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition. In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2014.01.041