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Synthesis and factorial design applied to a novel chitosan/sodium polyphosphate nanoparticles via ionotropic gelation as an RGD delivery system

•This works shows how to obtain chitosan nanoparticles with sodium polyphosphate.•Chitosan/polyphosphate nanoparticles were optimized using a 32 factorial design.•RGD-loaded chitosan/polyphosphate nanoparticles were not cytotoxic in Caco-2 cells. Chitosan nanoparticles have been extensively studied...

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Bibliographic Details
Published in:Carbohydrate polymers 2017-02, Vol.157, p.1695-1702
Main Authors: Kiilll, Charlene Priscila, Barud, Hernane da Silva, Santagneli, Sílvia Helena, Ribeiro, Sidney José Lima, Silva, Amélia M., Tercjak, Agnieszka, Gutierrez, Junkal, Pironi, Andressa Maria, Gremião, Maria Palmira Daflon
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Language:English
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Summary:•This works shows how to obtain chitosan nanoparticles with sodium polyphosphate.•Chitosan/polyphosphate nanoparticles were optimized using a 32 factorial design.•RGD-loaded chitosan/polyphosphate nanoparticles were not cytotoxic in Caco-2 cells. Chitosan nanoparticles have been extensively studied for both drug and protein/peptide delivery. The aim of this study was to develop an optimized chitosan nanoparticle, by ionotropic gelation method, using 32 full factorial design with a novel polyanion, sodium polyphosphate, well known under the trade name Graham salt. The effects of these parameters on the particle size, zeta potential, and morphology and association efficiency were investigated. The optimized nanoparticles showed an estimated size of 166.20±1.95nm, a zeta potential of 38.7±1.2mV and an efficacy of association of 97.0±2.4%. The Atomic Force Microscopy (AFM) and Scanning Electronic Microscopy (SEM) revealed spherical nanoparticles with uniform size. Molecular interactions among the components of the nanoparticles and peptide were evaluated by Fourier Transform Infrared Spectra (FTIR) and Differential Scanning Calorimetry (DSC). The obtained results indicated that, the developed nanoparticles demonstrated high biocompatible, revealing no or low toxicity in the human cancer cell line (Caco-2). In conclusion, this work provides parameters that contribute to production of chitosan nanoparticles and sodium polyphosphate with desirable size, biocompatible and enabling successful use for protein/peptides delivery.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2016.11.053