Synthesis of a chitosan-based photo-sensitive hydrogel and its biocompatibility and biodegradability

•Photo-sensitive azidehydroxyethyl chitosan (AZ-HECTS) was synthesized.•AZ-HECTS solution resulted in an insoluble hydrogel by 254nm UV irradiation for 90s.•AZ-HECTS hydrogels possessed favorable biocompatibility and biodegradability.•AZ-HECTS hydrogels were loaded with anticoagulant drugs heparin (...

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Bibliographic Details
Published in:Carbohydrate polymers 2017-06, Vol.166, p.228-235
Main Authors: He, Ming, Han, Baoqin, Jiang, Zhiwen, Yang, Yan, Peng, Yanfei, Liu, Wanshun
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biocompatible Materials - chemistry
Biodegradability
Cells, Cultured
Chitosan
Chitosan - chemistry
Drug Carriers - chemistry
Endothelial Cells - cytology
Heparin - administration & dosage
Hydrogel
Hydrogels
Photo-sensitive
Rats
Vascular Endothelial Growth Factor A
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Summary:•Photo-sensitive azidehydroxyethyl chitosan (AZ-HECTS) was synthesized.•AZ-HECTS solution resulted in an insoluble hydrogel by 254nm UV irradiation for 90s.•AZ-HECTS hydrogels possessed favorable biocompatibility and biodegradability.•AZ-HECTS hydrogels were loaded with anticoagulant drugs heparin (Hep).•AZ-HECTS-Hep hydrogels showed positive effect on HUVEC growth and VEGF expression. Hydroxyethyl chitosan (HECTS) is one of the most important water soluble derivatives of chitosan. In this study, photo-sensitive azidehydroxyethyl chitosan (AZ-HECTS) was synthesized with grafting degree 3.4%, and its water solution resulted in an insoluble hydrogel by 254nm UV irradiation for 90s. AZ-HECTS hydrogels, with water absorption 86.21%, had little impact on growth of mouse fibroblast (L929) and presented good cell biocompatibility. Obvious sudden degradation stage, slow degradation stage and no apparent toxicity was observed after AZ-HECTS hydrogels implanted into rats, and tissue inflammation was slighter, which indicated favorable biological safety. Furthermore, AZ-HECTS hydrogels was loaded with heparin, and released 50% of heparin accumulated 14d. AZ-HECTS-heparin hydrogels showed inhibitory effects on L929, and pro-growth functions within vascular endothelial cells seeded on the hydrogels, meanwhile a positive influence on vascular endothelial growth factor expression. In conclusion, AZ-HECTS hydrogels possessed favorable biocompatibility and biodegradability and had good potential as drug carrier.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2017.02.072