Self-assembled high molecular weight inulin nanoparticles: Enzymatic synthesis, physicochemical and biological properties

•HMW inulin enzymatically-synthesized is self-assembled into nanoparticles.•The assemblage of inulin nanoparticles is carried out during enzymatic synthesis.•The inulin forms nanoparticles at a critical aggregation concentration (CAC).•The CAC and nanoparticle size are influenced by the reaction tem...

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Bibliographic Details
Published in:Carbohydrate polymers 2019-07, Vol.215, p.160-169
Main Authors: Jiménez-Sánchez, Maira, Pérez-Morales, Rebeca, Goycoolea, Francisco M., Mueller, Monika, Praznik, Werner, Loeppert, Renate, Bermúdez-Morales, Víctor, Zavala-Padilla, Guadalupe, Ayala, Marcela, Olvera, Clarita
Format: Article
Language:English
Subjects:
Enzymatic synthesis
Fructan
Inulin
Inulosucrase
Polysaccharide nanoparticles
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Summary:•HMW inulin enzymatically-synthesized is self-assembled into nanoparticles.•The assemblage of inulin nanoparticles is carried out during enzymatic synthesis.•The inulin forms nanoparticles at a critical aggregation concentration (CAC).•The CAC and nanoparticle size are influenced by the reaction temperature.•The inulin nanoparticles are not toxic for peripheral blood mononuclear cells. Inulin has interesting physicochemical and functional properties, and therefore a wide range of applications in the food and medical industries. It has gained great traction due to its ability to form nanoparticles and its possible application as nanovehicle for drug delivery. In this work, we demonstrated that the enzymatically-synthesized high molecular weight (HMW) inulin forms stable spherical nanoparticles with an average diameter of 112 ± 5 nm. The self-assemblage of HMW inulin nanoparticles is carried out during enzymatic synthesis of the polymer, and become detectable after a certain critical aggregation concentration (CAC) is reached. Both, the CAC and nanoparticle size are influenced by the reaction temperature. These nanoparticles are not toxic for peripheral blood mononuclear cells, at concentrations below 200 μg/mL; no significant prebiotic potential was detected in cultures of 13 probiotic strains. This work contributes to a better understanding of the formation of HMW inulin nanoparticles and their biological properties.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2019.03.060