Facile formation of salecan/agarose hydrogels with tunable structural properties for cell culture

•Highly porous polysaccharide hydrogels were fabricated from salecan/agarose blends.•The structure of hydrogels can be tuned by changing polysaccharide content.•The salecan/agarose hydrogel was physically cross-linked.•The composite hydrogels supported cell adhesion and growth. Salecan polysaccharid...

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Bibliographic Details
Published in:Carbohydrate polymers 2019-11, Vol.224, p.115208, Article 115208
Main Authors: Qi, Xiaoliang, Su, Ting, Tong, Xianqin, Xiong, Wei, Zeng, Qiankun, Qian, Yuna, Zhou, Zaigang, Wu, Xuan, Li, Zhipeng, Shen, Liangliang, He, Xiaojun, Xu, Chuchu, Chen, Mengyu, Li, Yahui, Shen, Jianliang
Format: Article
Language:English
Subjects:
Agarose
Agrobacterium - chemistry
Animals
beta-Glucans - chemistry
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Cell culture
Cell Line
Cell Proliferation - drug effects
Hydrogel scaffolds
Hydrogels - chemistry
Hydrogen-Ion Concentration
Mice
Polysaccharide
Rheology
Salecan
Sepharose - chemistry
Tissue Engineering
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Summary:•Highly porous polysaccharide hydrogels were fabricated from salecan/agarose blends.•The structure of hydrogels can be tuned by changing polysaccharide content.•The salecan/agarose hydrogel was physically cross-linked.•The composite hydrogels supported cell adhesion and growth. Salecan polysaccharide produced by Agrobacterium sp. ZX09 is an attractive biopolymer to construct hydrogel scaffolds for cell culture. However, some limitations such as poor mechanical performance, complicated fabrication process and slow gelation times still exist in the biomedical applications of microbial-based salecan polysaccharide hydrogels. Here, a series of polysaccharide hydrogels composed of salecan and agarose with adjustable structural properties are designed. The resultant hybrid salecan/agarose hydrogels exhibit controllable physical and chemical properties including thermal stability, water uptake, mechanical strength and microarchitecture, which can be readily realized with minimum change of the polysaccharide content. Furthermore, cytotoxicity assays reveal that the designed composite hydrogels are non-toxic. More importantly, these hydrogels support cell survival, proliferation, and migration. Together, this work opens up a new avenue to build polysaccharide hydrogel platforms for tissue engineering.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2019.115208