Fabrication of oxidized bacterial cellulose by nitrogen dioxide in chloroform/cyclohexane as a highly loaded drug carrier for sustained release of cisplatin

• Published in: Carbohydrate polymers 2020-11, Vol.248, p.116745, Article 116745
• Main Authors: Solomevich, Sergey O., Dmitruk, Egor I., Bychkovsky, Pavel M., Nebytov, Alexander E., Yurkshtovich, Tatiana L., Golub, Natalia V.
• Format: Article
• Language: English
• Subjects: Adsorption; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; Carbohydrate Sequence; Cell Survival - drug effects; Cellulose, Oxidized - chemistry; Chloroform - chemistry; Cisplatin; Cisplatin - administration & dosage; Cisplatin - pharmacokinetics; Controlled release; Cyclohexanes - chemistry; Delayed-Action Preparations - administration & dosage; Delayed-Action Preparations - pharmacokinetics; Drug Carriers - chemistry; Drug delivery; Drug Delivery Systems - methods; Drug Liberation; HeLa Cells; Humans; Hydrogen-Ion Concentration; Kinetics; Microscopy, Electron, Scanning; Nitrogen dioxide; Nitrogen Dioxide - chemistry; Oxidized bacterial cellulose; Spectroscopy, Fourier Transform Infrared
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.116745

[Display omitted] •Oxidized bacterial cellulose (OBC) was prepared using dinitrogen tetroxide.•Carboxyl group content reaches high values and defines the OBC degradability.•Highly crystalline structure of OBC is preserved when using 10 % nitrogen dioxide.•High loading of cisplatin occurred in accordance with the Redlich-Peterson model.•Controlled release of cisplatin and degradation were confirmed. Carboxylated bacterial cellulose (OBC) was fabricated by oxidation with nitrogen dioxide in chloroform/cyclohexane and employed as a carrier for sustained release of antitumor substance cisplatin (CDDP). The influence of removing water method, solvent used in the synthesis, concentration of N2O4, and duration of the oxidation on content of carboxyl groups in reaction products was established. Due to the possibility of nitrogen dioxide to penetrate into cellulose crystallites, the carboxyl group content of the OBC reaches high values up to 4 mmol/g. In vitro degradation of OBC was determined under simulated physiological conditions. The immobilization of CDDP on OBC was studied in detail. The initial burst release of the drug from the polymer was depressed. The cytotoxicity of CDDP-loaded OBC was evaluated with HeLa cells. The unique structure and properties of OBC make it a great candidate as drug delivery carrier.