Factors determining self-assembly of hyaluronan

[Display omitted] •The network-forming and aggregation properties of hyaluronan were studied.•Effect of dissolution conditions and storage time was proved.•Specific changes in mesh density and aggregation rate were observed.•Structures on solid surface reflects properties of hyaluronan in solution....

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Published in:Carbohydrate polymers 2021-02, Vol.254, p.117307, Article 117307
Main Authors: Kocourková, Karolína, Musilová, Lenka, Smolka, Petr, Mráček, Aleš, Humenik, Martin, Minařík, Antonín
Format: Article
Language:English
Subjects:
Aggregates
Aluminum Silicates - chemistry
Atomic force microscopy
Drug Storage
Hyaluronan
Hyaluronic Acid - chemistry
Hydrogen Bonding
Hydrophobic and Hydrophilic Interactions
Microscopy, Atomic Force - methods
Molecular Structure
Molecular Weight
Network
Polymers - chemistry
Polysaccharides
Saline Solution, Hypertonic - chemistry
Solubility
Surface Properties
Water - chemistry
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Summary:[Display omitted] •The network-forming and aggregation properties of hyaluronan were studied.•Effect of dissolution conditions and storage time was proved.•Specific changes in mesh density and aggregation rate were observed.•Structures on solid surface reflects properties of hyaluronan in solution. The conditions determining network-forming and aggregation properties of hyaluronan on the mica surface were studied. The hyaluronan was deposited on the surface from aqueous and saline solutions and attached by a bivalent cation. The morphology of the immobilized assemblies was characterized by atomic force microscopy. The experimental results show that the morphology and size of the aggregates as well as the density of the interconnecting fibrillar network, both made of hyaluronan, at the liquid-solid phase interface are determined not only by its molecular weight or concentration in solution, but also by the dissolution conditions and storage time. These findings extend the current state of knowledge about the conformational variability of this biologically important polymer. Understanding the conformational variability is of great importance, as it governs the physiological functions of hyaluronan, as well as its processability and formulations. That in turn determines its usability in different pharmacological and biomaterial applications.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2020.117307