Cellulose nanofibers production using a set of recombinant enzymes

•Cellulose nanofibers (CNF) were produced combining hydrolytic and oxidative enzymes.•The set of recombinant enzymes was applied to sugarcane bagasse for the first time.•Enzymatically produced CNF are longer and more thermostable than TEMPO-oxidised CNF.•Enzymatic treatment using enzymes holds promi...

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Bibliographic Details
Published in:Carbohydrate polymers 2021-03, Vol.256, p.117510, Article 117510
Main Authors: Rossi, Bruno R., Pellegrini, Vanessa O.A., Cortez, Anelyse A., Chiromito, Emanoele M.S., Carvalho, Antonio J.F., Pinto, Lidiane O., Rezende, Camila A., Mastelaro, Valmor R., Polikarpov, Igor
Format: Article
Language:English
Subjects:
Biocatalysis
Biodegradation, Environmental
Cellulase - chemistry
Cellulose - chemistry
Cellulose nanofiber
Chlorides - chemistry
CNF
Cyclic N-Oxides - chemistry
Endo-1,4-beta Xylanases - chemistry
Enzyme
Green Chemistry Technology
Humans
Hydrolysis
Hydroxides - chemistry
Mixed Function Oxygenases - chemistry
Nanofibers - chemistry
Nanofibers - ultrastructure
Oxidation-Reduction
Polysaccharides - chemistry
Potassium Compounds - chemistry
Saccharum - chemistry
Sonication
Sugarcane bagasse
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Summary:•Cellulose nanofibers (CNF) were produced combining hydrolytic and oxidative enzymes.•The set of recombinant enzymes was applied to sugarcane bagasse for the first time.•Enzymatically produced CNF are longer and more thermostable than TEMPO-oxidised CNF.•Enzymatic treatment using enzymes holds promise for CNF production. Cellulose nanofibers (CNF) are renewable and biodegradable nanomaterials with attractive barrier, mechanical and surface properties. In this work, three different recombinant enzymes: an endoglucanase, a xylanase and a lytic polysaccharide monooxygenase, were combined to enhance cellulose fibrillation and to produce CNF from sugarcane bagasse (SCB). Prior to the enzymatic catalysis, SCB was chemically pretreated by sodium chlorite and KOH, while defibrillation was accomplished via sonication. We obtained much longer (μm scale length) and more thermostable (resisting up to 260 °C) CNFs as compared to the CNFs prepared by TEMPO-mediated oxidation. Our results showed that a cooperative action of the set of hydrolytic and oxidative enzymes can be used as a “green” treatment prior to the sonication step to produce nanofibrillated cellulose with advanced properties.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2020.117510