Study on a novel spherical polysaccharide from Fructus Mori with good antioxidant activity

• Published in: Carbohydrate polymers 2021-03, Vol.256, p.117516, Article 117516
• Main Authors: Yu-Hao, Dong, Chun, Chen, Qiang, Huang, Xiong, Fu
• Format: Article
• Language: English
• Subjects: Amidines - antagonists & inhibitors; Amidines - chemistry; Animals; Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Carbohydrate Sequence; Chemical Fractionation - methods; Complex Mixtures - chemistry; Conformation; Fructus Mori; Fruit - chemistry; Galactose - chemistry; Galactose - isolation & purification; Glucose - chemistry; Glucose - isolation & purification; Hexuronic Acids - chemistry; Hexuronic Acids - isolation & purification; Liver - drug effects; Liver - metabolism; Malondialdehyde - antagonists & inhibitors; Malondialdehyde - metabolism; Mannose - chemistry; Mannose - isolation & purification; Mice; Molecular Weight; Morus - chemistry; Oxidants - antagonists & inhibitors; Oxidants - chemistry; Plant Extracts - chemistry; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Structure
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.117516

[Display omitted] A novel polysaccharide (MFP1P) was isolated from Fructus Mori, followed by purification via DEAE-52 cellulose and 27 % ethanol fraction. The MFP1P had the molecular weight of 56.78 kDa and the total sugar content of 93.32±0.54 %. And the MFP1P is mainly composed of glucose, galactose, galacturonic acid and mannose with molar ratio of 66.62 %, 13.94 %, 18.24 % and 1.20 %, respectively. MFP1P was mainly composed of →3)-α-D-Gal (1→, β-D-Man-(1→ and →6)-α-D-Glc (1→ glycosidic bond and showed a spherical chain conformation with uniform distribution in solution. The MFP1P exhibited great antioxidant activity with oxygen-free radical absorption capacity (ORAC) values of 291.63±6.81 μmol TE/g and MDA IC50 of 0.289±0.022 mg/mL.