Electrospun polyamide-6/chitosan nanofibers reinforced nano-hydroxyapatite/polyamide-6 composite bilayered membranes for guided bone regeneration

• Published in: Carbohydrate polymers 2021-05, Vol.260, p.117769, Article 117769
• Main Authors: Niu, Xiaolian, Wang, Longfei, Xu, Mengjie, Qin, Miao, Zhao, Liqin, Wei, Yan, Hu, Yinchun, Lian, Xiaojie, Liang, Ziwei, Chen, Song, Chen, Weiyi, Huang, Di
• Format: Article
• Language: English
• Subjects: Animals; Bilayered tissue guided membranes; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Bone Regeneration - drug effects; Caprolactam - analogs & derivatives; Caprolactam - chemistry; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell Line; Cell Survival - drug effects; Chitosan - chemistry; Durapatite - chemistry; Mechanical properties; Membranes, Artificial; Mice; Nanofibers - chemistry; Nanostructures - chemistry; Nanostructures - toxicity; Osteoconductivity; Periodontal defect regeneration; Polymers - chemistry; Porosity; Seamlessly integrated layer structure; Surface Properties; Tensile Strength
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.117769

[Display omitted] •Electrospun PA6/CS nanofiber reinforced n-HA/PA6 bilayered composite was designed.•The composites are tightly bound and achieve seamlessly integrated layer structure.•Tensile strength of composites is above 1.75 times than that of n-HA/PA6 scaffolds.•Bilayered composite has biological safety, cytocompatibility and osteoconductivity. Periodontal defect poses a significant challenge in orthopedics. Guided Bone Regeneration (GBR) membrane is considered as one of the most successful methods applied to reconstruct alveolar bone and then to achieve periodontal defect repair/regeneration. In this paper, a novel polyamide-6/chitosan@nano-hydroxyapatite/polyamide-6 (PA6/CS@n-HA/PA6) bilayered tissue guided membranes by combining a solvent casting and an electrospinning technique was designed. The developed PA6/CS@n-HA/PA6 composites were characterized by a series of tests. The results show that n-HA/PA6 and electrospun PA6/CS layers are tightly bound by molecular interaction and chemical bonding, which enhances the bonding strength between two distinct layers. The porosity and adsorption average pore diameter of the PA6/CS@n-HA/PA6 membranes are 36.90 % and 22.61 nm, respectively. The tensile strength and elastic modulus of PA6/CS@n-HA/PA6 composites are 1.41 ± 0.18 MPa and 7.15 ± 1.09 MPa, respectively. In vitro cell culture studies demonstrate that PA6/CS@n-HA/PA6 bilayered scaffolds have biological safety, good bioactivity, biocompatibility and osteoconductivity.