Microfluidic platform for synthesis and optimization of chitosan-coated magnetic nanoparticles in cisplatin delivery

• Published in: Carbohydrate polymers 2021-08, Vol.265, p.118027, Article 118027
• Main Authors: Siavashy, Saeed, Soltani, M., Ghorbani-Bidkorbeh, Fatemeh, Fallah, Newsha, Farnam, Golrokh, Mortazavi, Seyed Alireza, Shirazi, Farshad H., Tehrani, Mohammad Hassan Houshdar, Hamedi, Mohammad Hossein
• Format: Article
• Language: English
• Subjects: Chitosan; Core-shell nanoparticles; Drug delivery; Magnetic nanoparticles; Microfluidic synthesis
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.118027

[Display omitted] •Preparation of chitosan-coated magnetic nanoparticles by microfluidic platform.•Development of integrated microfluidic device with precise and controllable mixing.•Comparison of microfluidics with batch flow manner in nanoparticle synthesis.•Microfluidics proved more efficiency in monodisperse nanoparticle synthesis.•Demonstration of more efficient cisplatin drug delivery by microfluidics. In this study, magnetic core/chitosan shell Nanoparticles (NPs) containing cisplatin were synthesized via cisplatin complexation with tripolyphosphate as the chitosan crosslinker using two different procedures: a conventional batch flow method and a microfluidic approach. An integrated microfluidic device composed of three stages was developed to provide precise and highly controllable mixing. The comparison of the results revealed that NPs synthesized in microchannels were monodisperse 104 ± 14.59 nm (n = 3) in size with optimal morphological characteristics, whereas polydisperse 423 ± 53.33 nm (n = 3) nanoparticles were obtained by the conventional method. Furthermore, cisplatin was loaded in NPs without becoming inactivated, and the microfluidic technique demonstrated higher encapsulation efficiency, controlled release, and consequently lower IC50 values during exposure to the A2780 cell line proving that microfluidic synthesized NPs were able to enter the cells and release the drug more efficiently. The developed microfluidic platform presents valuable features that could potentially provide the clinical translation of NPs in drug delivery.