Structure of the O-specific polysaccharide from the legume endosymbiotic bacterium Ochrobactrum cytisi strain ESC1T

The O-specific polysaccharide was obtained from the lipopolysaccharide of the legume-endosymbiotic bacterium Ochrobactrum cytisi strain ESC1T and studied by chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharide was found to have a disaccharide repeating unit containing α-d-fucose and...

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Bibliographic Details
Published in:Carbohydrate research 2015-09, Vol.413, p.37-40
Main Authors: Pac, Malgorzata, Komaniecka, Iwona, Zamlynska, Katarzyna, Turska-Szewczuk, Anna, Choma, Adam
Format: Article
Language:English
Subjects:
Fucose
Galactosamine
Lipopolysaccharide
Ochrobactrum cytisi
Pyruvyl
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Summary:The O-specific polysaccharide was obtained from the lipopolysaccharide of the legume-endosymbiotic bacterium Ochrobactrum cytisi strain ESC1T and studied by chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharide was found to have a disaccharide repeating unit containing α-d-fucose and β-N-acetyl-d-galactosamine residues connected via (1→3)-glycosidic bonds, resulting in the following structure: →3)-α-d-Fucp-(1→3)-β-d-GalpNAc-(1→ The d-GalpNAc residue was nonstoichiometrically substituted with a 4-O-methyl group (∼10%) or with a 4,6-O-(1-carboxy)-ethylidene residue (pyruvyl group) (∼10%). [Display omitted] •Structure of the O-polysaccharide of Ochrobactrum cytisi ESC1T was established.•OPS is built of disaccharide units composed of d-Fuc and d-GalNAc.•Galactosamine can be partly decorated with R-pyruvyl or O-methyl residues.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2015.05.002