Effects of cyclopenta[ c]phenanthrene and its derivatives on zona radiata protein, ERα, and CYP1A mRNA expression in liver of rainbow trout ( Oncorhynchus mykiss Walbaum)

• Published in: Chemico-biological interactions 2008-07, Vol.174 (1), p.60-68
• Main Authors: Woźny, Maciej, Brzuzan, Paweł, Łuczyński, Michał K., Góra, Maciej, Bidzińska, Joanna, Jurkiewicz, Paweł
• Format: Article
• Language: English
• Subjects: Cyclopenta[ c]phenanthrenes; CYP1A mRNA; Estrogen receptor α mRNA; Rainbow trout; Zona radiata protein mRNA
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2008.04.022

Despite cyclopenta-fused polycyclic aromatic hydrocarbons (CP-PAHs) have been detected in the environment, the ability of CP-PAH to induce cellular and tissue responses remains poorly characterized. In this study, xenoestrogen-associated responses (mRNA levels of estrogen receptor α, ERα, and zona radiata protein, Zrp) and xenobiotic effects (CYP1A mRNA) have been investigated in liver of juvenile rainbow trout after short-term treatment (8 and 24 h) with following compounds administered singly: cyclopenta[ c]phenanthrene (CP[ c]Ph); its derivatives, 5A-CP[ c]Ph; 5A6M-CP[ c]Ph; 5A9M-CP[ c]Ph; B[ c]Ph, a structurally similar polycyclic aromatic hydrocarbon; B[ a]P, a model CYP1A inducer; and zearalenone (ZEA), naturally occurring ligand for ER. The CYP1A mRNA expression after 24 h of exposure with CP[ c]Ph or its derivatives, except 5A9M-CP[ c]Ph, was 3–9-fold higher compared to controls ( P < 0.05), but it was less than that caused by B[ a]P (65-fold up regulation; P < 0.01). Moreover, neither of the CP-PAH compounds modulated liver ERα or Zrp mRNA levels as compared to effects associated with ZEA. Interestingly, a treatment with this ER-ligand, caused moderate but significant increase of CYP1A mRNA expression (about 2.5-fold; P < 0.05). The finding that ZEA is capable of acting as either estrogenic and xenobiotic compound, should be further explored in a more detailed and differently designed experiment.