Paris Saponin II induced apoptosis via activation of autophagy in human lung cancer cells

Paris Saponin II (PSII) has been shown anticancer activity against several cancer lines through the pro-apoptotic pathway. The aim of the study was to investigate the relationship between apoptosis and autophagy taking part in the anti-cancer mechanisms of PSII. In this study, PSII induced autophagy...

Full description

Saved in:
Bibliographic Details
Published in:Chemico-biological interactions 2016-06, Vol.253, p.125-133
Main Authors: Zhang, Lili, Man, Shuli, Wang, Yongshuai, Liu, Jing, Liu, Zhen, Yu, Peng, Gao, Wenyuan
Format: Article
Language:English
Subjects:
A549 Cells
Acetylcysteine - pharmacology
Apoptosis
Apoptosis - drug effects
Autophagy
Autophagy - drug effects
Blotting, Western
Cell Line
Chloroquine - toxicity
Glutathione - metabolism
Humans
JNK Mitogen-Activated Protein Kinases - metabolism
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Paris Saponin II
Phosphatidylinositol 3-Kinases - metabolism
Proto-Oncogene Proteins c-akt - metabolism
Reactive Oxygen Species - metabolism
Rhizome - chemistry
Rhizome - metabolism
ROS
Saponins - pharmacology
Signal Transduction - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Paris Saponin II (PSII) has been shown anticancer activity against several cancer lines through the pro-apoptotic pathway. The aim of the study was to investigate the relationship between apoptosis and autophagy taking part in the anti-cancer mechanisms of PSII. In this study, PSII induced autophagy and apoptosis in dose- and time-dependent manners. Meanwhile, it induced autophagy as early as 2 h after exposure to 1 μM of PSII accompanying with apoptosis. Blockade of autophagy with chloroquine (CQ) attenuated apoptosis, while regulation of reactive oxygen species (ROS) by N-acetyl cysteine (NAC), gallic acid (GA) and H2O2 could not influence autophagy. In addition, PSII induced apoptosis via activation of autophagy, which might be associated with the activation of JNK and inhibition of PI3K/AKT/mTOR pathway. All in all, our research increased the understanding of the role of PSII regulating autophagy and apoptosis, which would hopefully provide prospective strategies for cancer therapy. •Paris Saponin II induced apoptosis via activation of autophagy.•PSII induced cell apoptosis and autophagy in dose- and time-dependent manner.•PSII induced ROS-independent autophagy.
ISSN:0009-2797
1872-7786
DOI:10.1016/j.cbi.2016.05.016