Physiopathology of multiple organ dysfunctions in severely monocrotophos-poisoned rabbits

• Published in: Chemico-biological interactions 2017-12, Vol.278, p.9-14
• Main Authors: Hou, Yun-xiu, Liu, Shi-wen, Wang, Lian-wen, Wu, Shu-hua
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - metabolism; Animals; Insecticides - toxicity; Interleukin-1beta - blood; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Liver - drug effects; Liver - metabolism; Liver - pathology; Molecular damage; Monocrotophos - toxicity; Monocrotophos poisoning; Multiple organ dysfunction; Multiple Organ Failure - chemically induced; Multiple Organ Failure - pathology; Multiple Organ Failure - veterinary; Pathological injury; Rabbits; Radioimmunoassay; Thromboxane B2 - blood; Tumor Necrosis Factor-alpha - blood
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2017.08.016

Acute organophosphorus pesticides poisoning has a serious threat on people's health. This study aimed to investigate the pathogenesis and molecular mechanism of multiple organ dysfunction syndrome (MODS) in severely monocrotophos-poisoned rabbits. Chinchilla rabbits were used to build the monocrotophos-poisoned animal model via subcutaneous abdominal injection. Acetylcholinesterase activity was determined using the dithiobisnitrobenzoic acid enzyme kinetics method, and the free organophosphorus (FOP) toxic substances content was analyzed using the enzyme inhibition method. The contents of tumor necrosis factor (TNF-α), interleukin 1-β (IL-β) and thromboxane B2 (TXB2) in the plasma and tissue homogenates were determined via radioimmunoassay. Twenty-four hours after exposure, in comparison to the plasma, blood cells and homogenates of various tissues, the bile had a significantly different FOP content (P