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Acute exposure to the water-soluble fraction of gasoline (WSFG) affects oxygen consumption, nitrogenous-waste and Mg excretion, and activates anaerobic metabolism in the goldfish Carassius auratus
Contamination of aquatic environments by petroleum and its products (e.g. gasoline) is a hazard for aquatic organisms as a result of the potential toxicity of monocyclic aromatic hydrocarbons (BTEX) and polycyclic aromatic hydrocarbons (PAH). Our goal was to evaluate the acute effects of the water-s...
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| Published in: | Comparative biochemistry and physiology. Toxicology & pharmacology 2019-12, Vol.226, p.108590, Article 108590 |
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| container_title | Comparative biochemistry and physiology. Toxicology & pharmacology |
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| creator | G., Dal Pont Souza-Bastos, Luciana Rodrigues Giacomin, Marina Dolatto, Rafael Garrett Baika, Loana Mara Grassi, Marco Tadeu Ostrensky, Antonio Wood, Chris M. |
| description | Contamination of aquatic environments by petroleum and its products (e.g. gasoline) is a hazard for aquatic organisms as a result of the potential toxicity of monocyclic aromatic hydrocarbons (BTEX) and polycyclic aromatic hydrocarbons (PAH). Our goal was to evaluate the acute effects of the water-soluble fraction of gasoline (WSFG) on nitrogen excretion, osmoregulation, and metabolism of goldfish Carassius auratus. We first chemically characterized the WSFG and then tested its effects on these physiological aspects of C. auratus, in several different exposure scenarios (0, 0.25, 5, 10 and 25% of WSFG). The WSFG contained high concentrations BTEX (toluene 70% and benzene 17%) relative to PAH ( |
| doi_str_mv | 10.1016/j.cbpc.2019.108590 |
| format | article |
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[Display omitted]
•Exposure to WSFG inhibited aerobic metabolism and activated anaerobic metabolism in goldfish.•Urea-N and Mg2+ excretion increased, likely reflecting ATP breakdown•Ammonia excretion was not affected.•Routine O2 uptake rate (MO2) of goldfish was inhibited.•MO2 increased during post-exposure recovery.</description><identifier>ISSN: 1532-0456</identifier><identifier>EISSN: 1878-1659</identifier><identifier>DOI: 10.1016/j.cbpc.2019.108590</identifier><identifier>PMID: 31404698</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; BTEX ; Environmental pollution ; Gasoline - toxicity ; Goldfish - metabolism ; Ionic flux rates ; Lactate ; Magnesium - metabolism ; Mg2+ excretion ; Nitrogen - metabolism ; Oxygen - metabolism ; Oxygen Consumption ; Oxygen uptake ; PAH ; Polycyclic Aromatic Hydrocarbons - toxicity ; Urea-N ; Water - analysis ; Water Pollutants, Chemical - toxicity ; Water Pollution, Chemical</subject><ispartof>Comparative biochemistry and physiology. Toxicology & pharmacology, 2019-12, Vol.226, p.108590, Article 108590</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-582ab476ab480363e618784c237c2c0ac896cf8f6bd7dcd542693ec0491fc1c83</citedby><cites>FETCH-LOGICAL-c400t-582ab476ab480363e618784c237c2c0ac896cf8f6bd7dcd542693ec0491fc1c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31404698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>G., Dal Pont</creatorcontrib><creatorcontrib>Souza-Bastos, Luciana Rodrigues</creatorcontrib><creatorcontrib>Giacomin, Marina</creatorcontrib><creatorcontrib>Dolatto, Rafael Garrett</creatorcontrib><creatorcontrib>Baika, Loana Mara</creatorcontrib><creatorcontrib>Grassi, Marco Tadeu</creatorcontrib><creatorcontrib>Ostrensky, Antonio</creatorcontrib><creatorcontrib>Wood, Chris M.</creatorcontrib><title>Acute exposure to the water-soluble fraction of gasoline (WSFG) affects oxygen consumption, nitrogenous-waste and Mg excretion, and activates anaerobic metabolism in the goldfish Carassius auratus</title><title>Comparative biochemistry and physiology. Toxicology & pharmacology</title><addtitle>Comp Biochem Physiol C Toxicol Pharmacol</addtitle><description>Contamination of aquatic environments by petroleum and its products (e.g. gasoline) is a hazard for aquatic organisms as a result of the potential toxicity of monocyclic aromatic hydrocarbons (BTEX) and polycyclic aromatic hydrocarbons (PAH). Our goal was to evaluate the acute effects of the water-soluble fraction of gasoline (WSFG) on nitrogen excretion, osmoregulation, and metabolism of goldfish Carassius auratus. We first chemically characterized the WSFG and then tested its effects on these physiological aspects of C. auratus, in several different exposure scenarios (0, 0.25, 5, 10 and 25% of WSFG). The WSFG contained high concentrations BTEX (toluene 70% and benzene 17%) relative to PAH (<1%), and low levels of several metals (Al, Fe, Zn, Sr). Routine O2 uptake rate (MO2) of goldfish was inhibited by exposure to 5% WSFG, and during post-exposure recovery, MO2 increased in a dose-dependent fashion. Ammonia excretion was not affected by exposure to WSFG, but urea-N excretion increased progressively with the WSFG concentration. The same pattern of dose/response was observed for net Mg2+ loss rates and steadily increasing plasma lactate concentrations. Loss rates of Na+, Ca2+, K+ and Cl−, and plasma concentrations of Mg2+ and urea-N were not significantly altered. We propose that acute exposure to WSFG inhibits aerobic metabolism and activates anaerobic metabolism, breaking down ATP such that bound Mg2+ is liberated and the purine ring component is metabolized to urea-N, both of which are subsequently excreted.
[Display omitted]
•Exposure to WSFG inhibited aerobic metabolism and activated anaerobic metabolism in goldfish.•Urea-N and Mg2+ excretion increased, likely reflecting ATP breakdown•Ammonia excretion was not affected.•Routine O2 uptake rate (MO2) of goldfish was inhibited.•MO2 increased during post-exposure recovery.</description><subject>Animals</subject><subject>BTEX</subject><subject>Environmental pollution</subject><subject>Gasoline - toxicity</subject><subject>Goldfish - metabolism</subject><subject>Ionic flux rates</subject><subject>Lactate</subject><subject>Magnesium - metabolism</subject><subject>Mg2+ excretion</subject><subject>Nitrogen - metabolism</subject><subject>Oxygen - metabolism</subject><subject>Oxygen Consumption</subject><subject>Oxygen uptake</subject><subject>PAH</subject><subject>Polycyclic Aromatic Hydrocarbons - toxicity</subject><subject>Urea-N</subject><subject>Water - analysis</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Water Pollution, Chemical</subject><issn>1532-0456</issn><issn>1878-1659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9UcmO1DAQtRCIGQZ-gAPyESTS2InjdiQuoxazSIM4AOJoOZVyj1udOPIyy__xYTgEOHLx8vSWUj1CXnO24YzLD4cN9DNsasa7Aqi2Y0_IKVdbVXHZdk_Lu23qiolWnpAXMR4YY63g8jk5abhgQnbqlPw8h5yQ4sPsYw5Ik6fpFum9SRiq6I-5PyK1wUByfqLe0r0pqJuQvv3x9eLyHTXWIqRI_cPjHicKfop5nBf2ezq5FHxBfY7VvYklx0wD_bwvcRBw5SzI4n5XEmP5GQy-d0BHTKYvSXGkbvo9094fB-viLd2ZYGJ0udBzMCnHl-SZNceIr_7cZ-T7xadvu6vq5svl9e78pgLBWKpaVZtebGU5FGtkg3JZloC62UINzIDqJFhlZT9sBxhaUcuuQWCi4xY4qOaM1KsvBB9jQKvn4EYTHjVneqlEH_RSiV4q0WslRfRmFc25H3H4J_nbQSF8XAlYRr9zGHQEhxPg4EJZrR68-5__Lzbiofg</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>G., Dal Pont</creator><creator>Souza-Bastos, Luciana Rodrigues</creator><creator>Giacomin, Marina</creator><creator>Dolatto, Rafael Garrett</creator><creator>Baika, Loana Mara</creator><creator>Grassi, Marco Tadeu</creator><creator>Ostrensky, Antonio</creator><creator>Wood, Chris M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201912</creationdate><title>Acute exposure to the water-soluble fraction of gasoline (WSFG) affects oxygen consumption, nitrogenous-waste and Mg excretion, and activates anaerobic metabolism in the goldfish Carassius auratus</title><author>G., Dal Pont ; Souza-Bastos, Luciana Rodrigues ; Giacomin, Marina ; Dolatto, Rafael Garrett ; Baika, Loana Mara ; Grassi, Marco Tadeu ; Ostrensky, Antonio ; Wood, Chris M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-582ab476ab480363e618784c237c2c0ac896cf8f6bd7dcd542693ec0491fc1c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>BTEX</topic><topic>Environmental pollution</topic><topic>Gasoline - toxicity</topic><topic>Goldfish - metabolism</topic><topic>Ionic flux rates</topic><topic>Lactate</topic><topic>Magnesium - metabolism</topic><topic>Mg2+ excretion</topic><topic>Nitrogen - metabolism</topic><topic>Oxygen - metabolism</topic><topic>Oxygen Consumption</topic><topic>Oxygen uptake</topic><topic>PAH</topic><topic>Polycyclic Aromatic Hydrocarbons - toxicity</topic><topic>Urea-N</topic><topic>Water - analysis</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>Water Pollution, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>G., Dal Pont</creatorcontrib><creatorcontrib>Souza-Bastos, Luciana Rodrigues</creatorcontrib><creatorcontrib>Giacomin, Marina</creatorcontrib><creatorcontrib>Dolatto, Rafael Garrett</creatorcontrib><creatorcontrib>Baika, Loana Mara</creatorcontrib><creatorcontrib>Grassi, Marco Tadeu</creatorcontrib><creatorcontrib>Ostrensky, Antonio</creatorcontrib><creatorcontrib>Wood, Chris M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Comparative biochemistry and physiology. Toxicology & pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>G., Dal Pont</au><au>Souza-Bastos, Luciana Rodrigues</au><au>Giacomin, Marina</au><au>Dolatto, Rafael Garrett</au><au>Baika, Loana Mara</au><au>Grassi, Marco Tadeu</au><au>Ostrensky, Antonio</au><au>Wood, Chris M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute exposure to the water-soluble fraction of gasoline (WSFG) affects oxygen consumption, nitrogenous-waste and Mg excretion, and activates anaerobic metabolism in the goldfish Carassius auratus</atitle><jtitle>Comparative biochemistry and physiology. Toxicology & pharmacology</jtitle><addtitle>Comp Biochem Physiol C Toxicol Pharmacol</addtitle><date>2019-12</date><risdate>2019</risdate><volume>226</volume><spage>108590</spage><pages>108590-</pages><artnum>108590</artnum><issn>1532-0456</issn><eissn>1878-1659</eissn><abstract>Contamination of aquatic environments by petroleum and its products (e.g. gasoline) is a hazard for aquatic organisms as a result of the potential toxicity of monocyclic aromatic hydrocarbons (BTEX) and polycyclic aromatic hydrocarbons (PAH). Our goal was to evaluate the acute effects of the water-soluble fraction of gasoline (WSFG) on nitrogen excretion, osmoregulation, and metabolism of goldfish Carassius auratus. We first chemically characterized the WSFG and then tested its effects on these physiological aspects of C. auratus, in several different exposure scenarios (0, 0.25, 5, 10 and 25% of WSFG). The WSFG contained high concentrations BTEX (toluene 70% and benzene 17%) relative to PAH (<1%), and low levels of several metals (Al, Fe, Zn, Sr). Routine O2 uptake rate (MO2) of goldfish was inhibited by exposure to 5% WSFG, and during post-exposure recovery, MO2 increased in a dose-dependent fashion. Ammonia excretion was not affected by exposure to WSFG, but urea-N excretion increased progressively with the WSFG concentration. The same pattern of dose/response was observed for net Mg2+ loss rates and steadily increasing plasma lactate concentrations. Loss rates of Na+, Ca2+, K+ and Cl−, and plasma concentrations of Mg2+ and urea-N were not significantly altered. We propose that acute exposure to WSFG inhibits aerobic metabolism and activates anaerobic metabolism, breaking down ATP such that bound Mg2+ is liberated and the purine ring component is metabolized to urea-N, both of which are subsequently excreted.
[Display omitted]
•Exposure to WSFG inhibited aerobic metabolism and activated anaerobic metabolism in goldfish.•Urea-N and Mg2+ excretion increased, likely reflecting ATP breakdown•Ammonia excretion was not affected.•Routine O2 uptake rate (MO2) of goldfish was inhibited.•MO2 increased during post-exposure recovery.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31404698</pmid><doi>10.1016/j.cbpc.2019.108590</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Comparative biochemistry and physiology. Toxicology & pharmacology, 2019-12, Vol.226, p.108590, Article 108590 |
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| source | ScienceDirect Freedom Collection |
| subjects | Animals BTEX Environmental pollution Gasoline - toxicity Goldfish - metabolism Ionic flux rates Lactate Magnesium - metabolism Mg2+ excretion Nitrogen - metabolism Oxygen - metabolism Oxygen Consumption Oxygen uptake PAH Polycyclic Aromatic Hydrocarbons - toxicity Urea-N Water - analysis Water Pollutants, Chemical - toxicity Water Pollution, Chemical |
| title | Acute exposure to the water-soluble fraction of gasoline (WSFG) affects oxygen consumption, nitrogenous-waste and Mg excretion, and activates anaerobic metabolism in the goldfish Carassius auratus |
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