Roles of the genetic variation of alcohol-metabolizing enzymes on biomarkers in trauma patients with excessive alcohol intake at emergency department

Alcohol abuse has been implicated as an important factor for accidents. We evaluated the roles of different genetic combinations of the ADH2 and ALDH2 genotypes on biomarkers in trauma patients with excessive alcohol intake at our emergency department. Blood samples were obtained from 80 patients an...

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Bibliographic Details
Published in:Clinica chimica acta 2008-03, Vol.389 (1-2), p.14-18
Main Authors: Tseng, Yang-Ming, Jin, Yi-Ru, Chen, Ing-Jun, Huang, Fong-Dee, Wu, Szu-Hsien, Ma, Hsu, Chen, Sheng-Yi, Tsai, Li-Yu, Tsai, Shih-Meng, Lee, Jang-Hwa
Format: Article
Language:English
Subjects:
ADH2 and ALDH2
Adult
Aged
Alcohol
Alcohol Dehydrogenase - genetics
Alcoholic Intoxication - enzymology
Aldehyde Dehydrogenase - genetics
Biomarkers
Emergency department
Emergency Service, Hospital
Female
Genetic Variation
Humans
Male
Middle Aged
Polymorphism
Wounds and Injuries - enzymology
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Summary:Alcohol abuse has been implicated as an important factor for accidents. We evaluated the roles of different genetic combinations of the ADH2 and ALDH2 genotypes on biomarkers in trauma patients with excessive alcohol intake at our emergency department. Blood samples were obtained from 80 patients and 88 age-matched controls. The biomarkers, including AST, ALT, GGT, and MDA, were assayed. The polymerase chain reaction-restriction fragment length polymorphism method was used to determine the genetic polymorphisms of ADH2 and ALDH2. There were significant differences in the levels of AST, ALT, GGT, MDA, and AST/ALT ratios between the 2 groups. In addition, MDA values and AST/ALT ratios were significantly higher in the patients with normal activity of ADH2 than the patients with low activity of ADH2. Meanwhile, regarding ALDH2 genotypes, there were significantly higher ratios of AST/ALT in the patients with low activity of ALDH2. The highest AST/ALT ratios and MDA values were in the patients with ADH2 (⁎2/⁎2) and ALDH2 (⁎1/⁎2 and ⁎2/⁎2). In conclusion, our results indicated that alcohol-induced liver damage or oxidative stress might be influenced by the genetic variation of ADH2 or ALDH2. Therefore, the combinations of different ADH2 and ALDH2 genotypes may be influential markers for susceptibility to alcohol-induced liver damage.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2007.11.010