Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization

Terminal deoxynucleotidyl transferase (TdT) has been characterized as template-independent polymerase using single-stranded DNA (ssDNA) as primers to generate random oligonucleotides. However, the extension performance of TdT to single-stranded RNA (ssRNA) is vague. By systematically comparing and c...

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Bibliographic Details
Published in:Chinese chemical letters 2023-02, Vol.34 (2), p.107533, Article 107533
Main Authors: Han, Houyu, Cui, Jingyu, Zhou, Dianming, Hua, Deping, Peng, Weipan, Lin, Mengyao, Zhang, Yating, Li, Fangya, Gong, Xiaoqun, Zhang, Jianyu
Format: Article
Language:English
Subjects:
Polymerization
Single-stranded DNA
Single-stranded RNA
Template-independent
Terminal deoxynucleotidyl transferase
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Summary:Terminal deoxynucleotidyl transferase (TdT) has been characterized as template-independent polymerase using single-stranded DNA (ssDNA) as primers to generate random oligonucleotides. However, the extension performance of TdT to single-stranded RNA (ssRNA) is vague. By systematically comparing and contrasting the performance of TdT-catalyzed ssDNA and ssRNA extension, it is indicated that the catalytic efficiency of ssRNA as primers was about 3 times lower than ssDNA as primers. Collectively, it is believed that understanding the catalytic performance of TdT will help to design the strategy to synthesize chimeric DNA on 3′-OH of ssRNA, which becomes invaluable. [Display omitted] The performance of TdT-catalyzed ssDNA and ssRNA extension was contrasted, which will help to design the strategy to synthesize chimeric DNA on 3′-OH of ssRNA, which become invaluable.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2022.05.047