The metabolic versatility of PAOs as an opportunity to obtain a highly P-enriched stream for further P-recovery

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2015-06, Vol.270, p.459-467
• Main Authors: Acevedo, B., Camiña, C., Corona, J.E., Borrás, L., Barat, R.
• Format: Article
• Language: English
• Subjects: Enhanced biological phosphorus removal (EBPR); Glycogen accumulating metabolism (GAM); Phosphorus recovery; Polyphosphate (poly-P); Polyphosphate accumulating metabolism (PAM); Sequencing batch reactor (SBR)
• ISSN: 1385-8947; 1873-3212
• DOI: 10.1016/j.cej.2015.02.063

[Display omitted] •A simple process was proposed to recover P in a SBR operated for EBPR.•The P recovery did not affect the biological P removal performance.•This process enables to recover up to 81% of the incoming P per week. The effects of two sequencing batch reactor operation strategies for phosphorus stream enrichment over the biological phosphorus removal performance have been studied. The objective of both strategies is of performing an extraction cycle in order to obtain a new stream highly enriched with phosphorus. In the 1st strategy the amount of influent volatile fatty acids (VFAs) is the same in each cycle; while in the 2nd strategy the influent VFAs concentration is increased during phosphorus extraction experiments. Despite the strong decrease of the stored poly-P inside the cells in both strategies after the recovery cycles, the ability of the systems to remove phosphorus was not affected. The Prelease/HAcuptake ratio (changing from 0.73 to 0.21mmolPmmolC−1) together with FISH analyses (around 85% of Accumulibacter through the experimental period) confirmed that a shift from PAM to GAM occurred after phosphorus enrichment in the 2nd strategy experiments. These results suggest that energy required for VFA uptake by polyphosphate-accumulating organisms (PAOs) was not only derived from polyphosphates degradation, but also from glycogen degradation. FISH also revealed that Type II Accumulibacter species are responsible of the metabolic shift. The strategy based on increasing influent VFAs concentration during phosphorus extraction experiments showed a higher extraction efficiency (from 46% to 76%), as higher phosphorus concentration within supernatant can be achieved (from 113.9 to 198.7mgPl−1). Following this strategy, it is possible to concentrate up to 81% of the incoming phosphorus in a single enriched stream. This suggests that, despite the extra addition of carbon source needed (9%), this strategy is more appropriate if phosphorus recovery for reuse purposes is required.