Performance and bacterial community dynamics of a CANON bioreactor acclimated from high to low operational temperatures

•Acclimation of a CANON bioreactor from 35°C to 15°C was accomplished.•Performance at 15°C could not meet nitrogen discharge standards.•AOB dominance went from Nitrosomonas to Prosthecobacter during acclimation.•Acclimation drove a shift in anammox dominance from Brocadia to Anammoxoglobus.•Rhodanob...

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Bibliographic Details
Published in:Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2016-03, Vol.287, p.557-567
Main Authors: Gonzalez-Martinez, Alejandro, Rodriguez-Sanchez, Alejandro, Garcia-Ruiz, Maria Jesus, Muñoz-Palazon, Barbara, Cortes-Lorenzo, Carmen, Osorio, Franscisco, Vahala, Riku
Format: Article
Language:English
Subjects:
Anammox
CANON
Low temperature
Nitrogen removal
Pyrosequencing
Wastewater treatment
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Summary:•Acclimation of a CANON bioreactor from 35°C to 15°C was accomplished.•Performance at 15°C could not meet nitrogen discharge standards.•AOB dominance went from Nitrosomonas to Prosthecobacter during acclimation.•Acclimation drove a shift in anammox dominance from Brocadia to Anammoxoglobus.•Rhodanobacter was the only NOB found and proliferated only at low temperature. In this study, a lab-scale CANON bioreactor was operated for 260days, decreasing operational temperature from 35°C to 15°C and from 466 to 100mg-NL−1 ammonium. This was done in order to check the feasibility of the acclimation of CANON biomass treating anaerobic digestion supernatant to B-stage influent wastewater operational conditions. Results showed that decrease in temperature posed an impact over the performance of the CANON bioreactor as well as over its bacterial assemblage. Nitrogen removal efficiency showed a moderate decrease when the system was acclimated from 35°C to 25°C, but it decreased dramatically in the acclimation from 25°C to 15°C. The decrease in temperature and influent ammonium concentration posed an impact over the bacterial community structure of the system. Ammonium oxidizing bacteria changed from Nitrosomonas, Nitrosospira or Nitrosovibrio genera at high temperature and influent ammonium to Prosthecobacter at low temperature and low ammonium. As well, dominant anammox bacteria genus changed from Candidatus Brocadia to Candidatus Anammoxoglobus during acclimation. Proliferation of nitrite oxidizing bacteria only occurred under low temperature and influent ammonium conditions with growth of Rhodanobacter genus.
ISSN:1385-8947
1873-3212
DOI:10.1016/j.cej.2015.11.081