In situ production and renewal of biocatalytic coatings for use in enzymatic reactive distillation

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2016-12, Vol.306, p.992-1000
• Main Authors: Heils, Rene, Hu, Xihua, Liese, Andreas, Smirnova, Irina
• Format: Article
• Language: English
• Subjects: Catalyst exchange; Catalytic packing; Enzymatic reactive distillation; Enzyme immobilization; Sol-gel coating
• ISSN: 1385-8947; 1873-3212
• DOI: 10.1016/j.cej.2016.08.031

[Display omitted] •Enzymes in reactive distillation are useful for the production of chiral products.•Sol-gel coating enables immobilization of enzymes on structured packings.•Coating procedure was carried out in situ by flooding the column with reacting sol.•Expensive refilling of catalyst bags and installation of new packings is omitted.•In situ variation of position and length of reactive section was demonstrated. Reactive distillation is one of the most well-established integrative separation processes in chemical industry which is used for the production of e.g. fuel additives. Recently, the feasibility of enzymes in reactive distillation columns was demonstrated, which possibly extends the scope towards more selective reactions with chiral products. However, the low thermal stability of enzymes necessitates a more frequent catalyst exchange which is usually a knock-out criterion for this process due to very high costs of catalytic structures and long process shut down times. In this work, an in situ production method for biocatalytic coatings on structured packings is presented which enables production and renewal of the catalytic structures in place, i.e. without disassembly of the column internals. By flooding the column with reacting enzyme/silica-sol, gauze structured packings are covered with a uniform layer of gel, which after drying results in a catalytic coating containing immobilized enzymes. The in situ method could help simplify the catalyst exchange in reactive distillation processes and furthermore allows changes in the reactive section in terms of amount and position of the catalyst which are important process parameters in the design phase of reactive distillation processes. Video: For a video showing the in situ procedure, please see Supplementary data.