Titania nanotube array supported nanoceria with redox cycling stability ameliorates oxidative stress-inhibited osteogenesis

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2021-07, Vol.415, p.128913, Article 128913
• Main Authors: Shao, Dandan, Li, Kai, Hu, Tao, Wang, Shanjin, Xu, Haowei, Zhang, Shubao, Liu, Shiwei, Xie, Youtao, Zheng, Xuebin
• Format: Article
• Language: English
• Subjects: Antioxidant enzyme-like property; Cerium oxide nanoparticles; Osteoporosis; Oxidative stress; Titania nanotube array
• ISSN: 1385-8947; 1873-3212
• DOI: 10.1016/j.cej.2021.128913

[Display omitted] •TiNTA-CeNPs retained the cycling capability of Ce4+/Ce3+ in H2O2-containing PBS.•Preferable phosphate adsorption on Ti3+ in TiNTA-CeNPs preserved Ce3+ active sites.•Relationship between electronic structure and enzyme-like activity was established.•TiNTA-CeNP2 exhibited greater osteoprotective effect under oxidative stress. The core catalytic property of cerium oxide nanoparticles (CeNPs) has been proposed to be due to the redox cycling capability of Ce4+/Ce3+ oxidation state. Thus far there are few reports on controlling the cycling stability of Ce4+/Ce3+ couple in biological fluids which is the most important parameter in the application of CeNPs as a regenerative nano-antioxidant. In this study, vertically aligned titania nanotube array supported CeNPs (TiNTA-CeNPs), regardless of the pre-dominant Ce oxidation state, retained the cycling capability of Ce4+/Ce3+ in H2O2-containing phosphate-buffered saline (PBS) compared with Ti supported CeNPs. This was because preferable phosphate adsorption on surface Ti3+ in TiNTA-CeNPs preserved Ce3+ active sties. Accordingly, Ce3+-rich TiNTA-CeNP1 and Ce4+-rich TiNTA-CeNP2 in PBS exhibited more sustained superoxide dismutase and catalase mimetic response, respectively. A correlation between electronic band structures of Ce-Ti mixed oxides and redox potential of reactive oxygen species (ROS) aided in interpretation of enhanced redox cycling capability and enzyme-like activities. Less Fenton-active TiNTA-CeNP2 exhibited a greater ability to protect pre-osteoblasts against ROS-induced oxidative stress in vitro. To demonstrate in vivo osteoprotective effect of TiNTA-CeNP2, a rat model of oxidative stress-related osteoporosis was established. The results indicated that TiNTA-CeNP2 held great potential for ameliorating osteogenesis in oxidative stress-related bone diseases.