A novel bio-based film-forming helper derived from Leuconostoc mesenteroides: A promising alternative to chemicals for the preparation of biomass film

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2024-08, Vol.493, p.152436, Article 152436
• Main Authors: Chen, Yukun, Gong, Xiaofang, Song, Jie, Peng, Yinan, Zeng, Yang, Chen, Juan, Wang, Zhiye, Li, Zhilong, Zhu, Ying
• Format: Article
• Language: English
• Subjects: Biomass film; Film-forming helper; Leuconostoc mesenteroides; Performance evaluation; Waste resource utilization
• ISSN: 1385-8947
• DOI: 10.1016/j.cej.2024.152436

[Display omitted] •A new bio-based film-forming helper (BFH-L) had been successfully developed.•Leuconostoc mesenteroides’ genome offered proof that BFH-L promotes plant growth.•BFH-L has the potential to replace chemical film-forming helpers in liquid mulch.•The triangle model was used to optimize the preparation process of biomass film.•Cow dung was successfully transformed into biomass film by BFH-L. Some microorganisms produce biopolymers with adhesive, film-forming, and plant-promoting properties in their fermentation broths. These biopolymers hold significant potential as substitutes for chemical film-forming helpers in liquid mulch. The present study involved the preparation of a novel bio-based film-forming helper (BFH-L) through the process of bio-fermentation utilizing Leuconostoc mesenteroides. The successful preparation of BFH-L was confirmed through the assessment of adhesive properties and plant growth-promoting efficacy exhibited by L. mesenteroides and BFH-L. The comprehensive genomic analysis of L. mesenteroides provided compelling evidence supporting the effectiveness of BFH-L, which can be attributed to the functional contribution of genes encoding extracellular glycoside polymers from L. mesenteroides. Through the evaluation and comparison of a triangle model, BFH-L can effectively replace chemical film-forming helpers in the preparation of biomass film (BF). The optimal performance of BF was achieved when 3.0 g/600 mL of BFH-L and 30 g/600 mL of cow dung were added. BF exhibited exceptional water retention ability (water loss: 38.00 ± 3.46 %), superior plant growth promotion effect (germination rate: 100.00 ± 0 %, plant growth: 0.34 ± 0.03 g), and minimal environmental impact (pH value = 8.7). This research provides a new strategy for reducing plastic film pollution while achieving green recycling of organic solid waste from agriculture and animal husbandry.