Effects of insulin-like growth factor-1 on okadaic acid-induced apoptosis in SH-SY5Y cells

• Published in: Cell biology international 2005-09, Vol.29 (9), p.803-808
• Main Authors: Xing, Changhong, Peng, Ying, Chang, Rui, Yin, Yanling, Xie, Zuoping
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Annexin A5; Apoptosis; Apoptosis - drug effects; Caspase; Cell Line, Tumor; Cell Survival - drug effects; Dose-Response Relationship, Drug; Drug Interactions; Enzyme Inhibitors - pharmacology; Flow Cytometry; Humans; Indicators and Reagents; Insulin-Like Growth Factor I - pharmacology; Insulin-like growth factor-1; Neurons - drug effects; Okadaic acid; Okadaic Acid - pharmacology; Propidium
• ISSN: 1065-6995; 1095-8355
• DOI: 10.1016/j.cellbi.2005.04.012

The effects of insulin-like growth factor-1 (IGF-1) on the cytotoxicity and apoptosis induced by okadaic acid (OA) in SH-SY5Y cells were investigated. Cell viability was measured using the MTT (3-(4,5-dimethylthiazolyl-2)-2,-5-diphenyltetrazolium bromide) assay. Early and late apoptosis/necrosis were analyzed by flow cytometry using Annexin V and propidium iodide (PI) double-staining. Caspase-3 activation was detected by Western blot analysis. Preincubation with IGF-1 for 24 h prevented cytotoxicity induced by 40 nM OA given for 24 h, and the MTT value significantly increased. Incubation with 20 nM OA for 24 h caused a marked increase in the percentage of early apoptotic and late apoptotic/necrotic cells, which was not dependent on the activation of caspase-3. OA-induced apoptosis was significantly decreased by pretreatment with 10 ng/ml of IGF-1 for 24 h. The results supported the hypothesis that IGF-1 may be useful in the treatment of Alzheimer's disease.