Environmental biomonitoring of essential and toxic elements in human scalp hair using accelerated microwave-assisted sample digestion and inductively coupled plasma optical emission spectroscopy

• Published in: Chemosphere (Oxford) 2017-05, Vol.174, p.708-715
• Main Authors: Kumakli, Hope, Duncan, A'ja V., McDaniel, Kiara, Mehari, Tsdale F., Stephenson, Jamira, Maple, Lareisha, Crawford, Zaria, Macemore, Calvin L., Babyak, Carol M., Fakayode, Sayo O.
• Format: Article
• Language: English
• Subjects: Adult; Aged; Analytic Sample Preparation Methods - methods; Environmental Monitoring - methods; Environmental Pollutants - antagonists & inhibitors; Environmental Pollutants - toxicity; Environmental-biomonitoring; Female; Hair - chemistry; Human-scalp-hair; Humans; Inductively-coupled-plasma-optical-emission-spectroscopy; Inter-element-association; Kinetics; Limit of Detection; Male; Microwaves; Middle Aged; Scalp - chemistry; Smoking; Smoking-habits; Spectrophotometry, Atomic; Tobacco Products; Trace Elements - analysis; Trace-element
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.02.032

Human scalp hair samples were collected and used to assess exposure to toxic elements and essential elements in the state of North Carolina, USA using accelerated microwave assisted acid digestion and inductively coupled plasma optical emission spectroscopy (ICP-OES). The figures-of-merit of the ICP-OES were appropriate for elemental analysis in scalp hair with detection limits as low as 0.0001 mg/L for Cd, good linearity (R2 > 0.9978), and percent recoveries that ranged from 96 to 106% for laboratory-fortified-blanks and 88–112% for sample spike recovery study. The concentrations of essential elements in scalp hair were larger than those of toxic elements, with Ca having the highest average concentration (3080 μg/g, s = 14,500, n = 194). Some of the maximum concentrations observed for As (65 μg/g), Ni (331 μg/g), Cd (2.96 μg/g), and Cr (84.6 μg/g) in individual samples were concerning, however. Samples were statistically analyzed to determine the influence of race, gender, smoking habits, or age on the elemental concentrations in scalp hair. Higher concentrations of essential elements were observed in the scalp hair of Caucasians, females, and non-smokers, and the differences were often significant at a 90% confidence level. Several pairs of essential elements, for example Ca-K, Ca-Mg, and Ca-Zn, were strongly correlated in Caucasian hair but uncorrelated in African-American hair. Similarly, essential elements were strongly correlated in female hair but weakly correlated in male hair. Toxic element pairs (As-Cd, As-Se, Pb-As, and Se-Cd) were strongly correlated in the hair of smokers but uncorrelated in that of non-smokers, suggesting that cigarette smoke is a common source of toxic elements in humans. [Display omitted] •Scalp hair was used to assess human exposure to toxic elements in North Carolina, USA.•High levels of As, Ni, Se, Pb and Cr were detected in many samples.•As-Cd, As-Se, Pb-As and Se-Cd highly correlated in the hair of smokers but not in non-smokers.•The race, gender, die and smoking habits influenced elemental profiles in human scalp hair.