Hepatotoxicity and metabolic effects of cellular extract of cyanobacterium Radiocystis fernandoi containing microcystins RR and YR on neotropical fish (Hoplias malabaricus)

• Published in: Chemosphere (Oxford) 2017-05, Vol.175, p.431-439
• Main Authors: Paulino, Marcelo Gustavo, Rossi, Priscila Adriana, Venturini, Francine Perri, Tavares, Driele, Elisabete da Silva Souza, Naiara, Sakuragui, Marise Margareth, Moraes, Gilberto, Terezan, Ana Paula, Fernandes, João Batista, Giani, Alessandra, Fernandes, Marisa Narciso
• Format: Article
• Language: English
• Subjects: Alanine Transaminase - blood; Animals; Aspartate Aminotransferases - blood; Bilirubin; Bilirubin - blood; Characiformes - blood; Characiformes - metabolism; Cyanobacteria - chemistry; Energy Metabolism - drug effects; Glycogen - metabolism; Hepatocytes - drug effects; Hepatocytes - enzymology; Hepatocytes - ultrastructure; Intermediate metabolism; Liver - drug effects; Liver - enzymology; Liver - ultrastructure; Liver damage; Microcystins - isolation & purification; Microcystins - toxicity; Muscles - drug effects; Muscles - metabolism; Phosphatases; Transaminases
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.02.089

The toxicological effect of cellular extract of cyanobacterium Radiocystis fernandoi strain R28 containing RR and YR microcystins was analyzed in the fish Hoplias malabaricus with emphasis on the liver structure and energetic metabolism, after short-term exposure. Fish were intraperitoneally (i.p.) injected with 100 μg of equivalent MC-LR kg−1 body mass containing in the cellular extract of R. fernandoi strain R28. Twelve and 96 h post-injection, the plasma, liver and white muscle were sampled for biochemical analyses and liver was also sampled for morphological analyses. After i.p. injection, the activity of acid phosphatase (ACP), alanine aminotransferase (ALT) and direct bilirubin increased in the plasma, while ALT and aspartate aminotransferase (AST) decreased in the liver. Glucose, lactate and pyruvate increased while protein decreased in the plasma; glycogen, pyruvate and lactate decreased in the liver; and glycogen and glucose increased in the muscle. Ammonia increased in the plasma, liver and muscle. The hepatocyte cell shape changed from polyhedral to round after cellular extract injection; there was loss of biliary canaliculus organization, but the biliary duct morphology was conserved in the liver parenchyma. In conclusion, microcystins present in the cellular extract of R. fernandoi strain R28 affect the liver structure of H. malabaricus, but the liver was able to continuously produce energy by adjusting its intermediate metabolism; glycogenolysis and gluconeogenesis maintained glucose homeostasis and energy supply. [Display omitted] •Microcystins RR and YR are majority toxins produced by R. fernandoi strain R28.•Cyanobacterial cellular extract changes the plasma and liver enzyme activity in fish.•Biliary canaliculus is altered, but biliary duct integrity is maintained.•Glycogen depletion occurred in liver and increased in white muscles.•Energetic metabolic compounds are altered in liver, plasma and muscles.