Microbial communities and functional genes of nitrogen cycling in an electrolysis augmented constructed wetland treating wastewater treatment plant effluent

To enhance nitrogen removal efficiency, a new electrolysis augmented constructed wetland (E-CW) was applied for nitrogen removal from waste water treatment plant (WWTP) effluent. This work demonstrated that E-CW could remove NO3− efficiently (45.5%–83.4%) under low CCOD/N ratio (average 2.29 ± 0.45)...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2018-11, Vol.211, p.25-33
Main Authors: Zhang, Pengfei, Peng, Yuke, Lu, Junling, Li, Jie, Chen, Huiping, Xiao, Lin
Format: Article
Language:English
Subjects:
Bacterial community
Denitrification
Electrolysis augmented constructed wetland
Functional gene group
Network analysis
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Summary:To enhance nitrogen removal efficiency, a new electrolysis augmented constructed wetland (E-CW) was applied for nitrogen removal from waste water treatment plant (WWTP) effluent. This work demonstrated that E-CW could remove NO3− efficiently (45.5%–83.4%) under low CCOD/N ratio (average 2.29 ± 0.45) with little amount of NH4+ and NO2− generation. High throughput 16S rRNA sequence analysis revealed that Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, and Verrucomicrobia were the dominant phyla in the E-CW. However, abundance of denitrifiers and denitrification genes decreased along with the operation of E-CW. Four functional gene pairs of anammox-amoA, (narG + napA)-(nirK + nirS), (narG + napA)-nosZ and qnorB-nosZ showed positive correlations with each other. Co-occurrence network analysis results indicated that functional guilds of FeOB and FeRB coupled with denitrifiers and contributed to the process of nitrogen removal in the E-CW. Overall, this work illustrated E-CW was a feasible and effective technology for enhancing nitrogen removal, and provided a theoretical basis for better design and operation of E-CW. •Electrolysis augmented CW could remove NO3− efficiently under low C/N.•The abundance of denitrifiers and genes of narG, napA, qnorB and nosZ decreased during the operation of E-CW.•Cooperative anammox and denitrifiers played a pivotal role in nitrogen removal in E-CW.•The coupling of denitrifier with FeOB and FeRB contributed to nitrogen removal in E-CW.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2018.07.067