Bacterial peroxidase-mediated enhanced biodegradation and mineralization of bisphenol A in a batch bioreactor

• Published in: Chemosphere (Oxford) 2019-05, Vol.222, p.549-555
• Main Authors: Moussavi, Gholamreza, Abbaszadeh Haddad, Fatemeh
• Format: Article
• Language: English
• Subjects: Biodegradation; Bisphenol A; Enzyme; Mineralization; Peroxidase
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2019.01.190

The present study focused on the enhanced biodegradation and mineralization of bisphenol A (BPA) as a toxic endocrine disrupting compound using peroxidase-mediated bioprocess under H2O2-infusion. The complete biodegradation of 100 mg/L BPA was achieved within 54 h reaction time at the optimum H2O2:BPA molar ratio of 10. BPA concentrations up to 100 mg/L had no inhibitory effect on the bacterial biomass at which a dehydrogenase activity of 9.1 μg TF/gbiomass and a peroxidase activity of 1.4 U/mL was obtained. The increase in biomass concentration from 90 to 450 mg/L improved the BPA biodegradation from 70.3% to 97.8% and its mineralization from 11.5% to 71.2% at the reaction time of 36 h. The highest BPA biodegradation rate was found to be 10.8 mg BPA/gbiomass. h. Accordingly, infusing H2O2 into the bioreactor stimulated the bacteria to produce peroxidase and allowed peroxidase-mediated enhanced biodegradation of BPA. •Peroxidase-mediated enhanced biodegradation and mineralization of BPA was tested.•High amount of bacterial peroxidase was produced in-situ.•Complete biodegradation of BPA was achieved within 54 h.•Specific BPA removal rate was much greater than that in the conventional bioreactors.•Peroxidase-mediated biodegradation is an advanced remediation bioprocess.