Mechanistic insight for DBP induced growth inhibition in Vigna radiata via oxidative stress and DNA damage

• Published in: Chemosphere (Oxford) 2021-01, Vol.263, p.128062, Article 128062
• Main Authors: Ranjan, Jyoti, Mandal, Tamal, Mandal, Dalia Dasgupta
• Format: Article
• Language: English
• Subjects: Antioxidant enzymes; Disinfectants - toxicity; Disinfection; Disinfection by-products (DBPs); DNA Damage; Genotoxicity; Hydrogen Peroxide; Oxidative Stress; Phytotoxicity; ROS level; Vigna - genetics; Vigna radiata
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2020.128062

Chlorination is important to the safeness of recouped water; though it shows concern about disinfection by-products (DBPs) formation and its toxic effects. DBPs generation mostly specified by category of disinfectant utilized and naturally occurring organic matter present in the water pre and post disinfection. Plants are exposed to diverse stresses of environment across their lifespan. Reactive oxygen species (ROS) perform significant roles in preserving ordinary plant growth and enhancing their tolerance towards stress. This study is focused on the generation and elimination of ROS in apical meristematic growth and responses in Vigna radiata towards DBPs exposure. Phytotoxic and genotoxic effect of selected DBPs, TCAA (trichloroacetic acid), TCM (trichloromethane), TBM (tribromomethane) revealed concentration-dependent root length inhibition, germination index, vigour index, tolerance index, root/shoot ratio with higher EC50 value for TCM (6000 mg/L, 50.26 mM) over TCAA and TBM (1850 mg/L, 11.32 mM; 4000 mg/L, 15.83 mM). DNA laddering assay demonstrated DBP induced DNA damage to be concentration-dependent too. The concentration-dependent increase in the lipid peroxidation, H2O2 generation for each DBPs examined with highest oxidative stress for TCAA over TBM and TCM at fixed concentration illustrates that possible mechanism behind observed toxicity may be via ROS. Its regulation by antioxidative defense enzymes activities can be attributed to observed decline in these enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase) activities with increasing concentration again where TCAA found more significantly affected than TBM and TCM over control. Results thus provide a useful understanding of the mechanism of DBP induced phytotoxicity and genotoxicity in V.radiata. •Tolerance potential of Vigna radiata towards DBPs (at higher concentrations).•Found that Haloacetic acid (TCAA) is more toxic than trihalomethanes (TCM and TBM).•Mechanistic insight for DBP induced root growth inhibition via ROS.•Due to ROS generation protein content is decreased.•Antioxidant defense enzymes played a vital role in tolerance towards DBPs.