Femtosecond laser-induced dissociation (fs-LID) as an activation method in mass spectrometry

We report the photolysis of biomolecules in a Fourier-transform ion cyclotron resonance mass spectrometer by intense near-infrared femtosecond laser pulses. Photo-fragmentation was accompanied by photo-ionization and created a large number and variety of charged fragments, which could be identified...

Full description

Saved in:
Bibliographic Details
Published in:Chemical physics 2018-10, Vol.514, p.106-112
Main Authors: Neidel, Christian, Kuehn, Andreas, Schulz, Claus Peter, Hertel, Ingolf V., Linscheid, Michael W., Schultz, Thomas
Format: Article
Language:English
Subjects:
Femtosecond laser
Fs-LID
FT-MS
Mass spectrometry
Peptides
Proteins
Sequencing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report the photolysis of biomolecules in a Fourier-transform ion cyclotron resonance mass spectrometer by intense near-infrared femtosecond laser pulses. Photo-fragmentation was accompanied by photo-ionization and created a large number and variety of charged fragments, which could be identified with high confidence due to the outstanding resolving power and mass accuracy of the mass spectrometer. Fragmentation patterns were sufficient for peptide sequence analysis. Fragments formed by non-ergodic cleavage retained labile post-translational modifications.
ISSN:0301-0104
DOI:10.1016/j.chemphys.2018.05.008