Rapid and sensitive determination of anthraquinones in Chinese herb using 1-butyl-3-methylimidazolium-based ionic liquid with β-cyclodextrin as modifier in capillary zone electrophoresis

• Published in: Journal of Chromatography A 2004-12, Vol.1059 (1-2), p.191-198
• Main Authors: Qi, Shengda, Cui, Shuya, Chen, Xingguo, Hu, Zhide
• Format: Article
• Language: English
• Subjects: 1-Butyl-3-methylimizolium tetrafluoroborate; Anthraquinones; Anthraquinones - analysis; beta-Cyclodextrins - chemistry; Biological and medical sciences; Borates - chemistry; Capillary zone electrophoresis; Drugs, Chinese Herbal - chemistry; Electrophoresis, Capillary - methods; General pharmacology; Imidazoles - chemistry; Ionic liquids; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Sensitivity and Specificity; β-Cyclodextrin
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.09.090

The present study reported the ionic liquid (IL) used as running electrolyte in capillary zone electrophoresis (CZE) with β-cyclodextrin (β-CD) as modifier for the separation of anthraquinones extract of Chinese herb Paedicalyx attopevensis Pierre ex Pitard. The optimum running electrolyte was 60mM 1-butyl-3-methylimizolium tetrafluoroborate (1B-3MI-TFB) solution with 4.0mM β-CD. The pH was 10.00 and the applied voltage was 20kV with detection at 254nm. The present method was compared with others and the effect of Joule heating was discussed as well. More significantly, this method is the development of the ionic liquids application to the capillary electrophoresis.