Gas chromatographic method for the analysis of allelopathic natural products in rye ( Secale cereale L.)

Accurate and reproducible methods for the analysis of plant allelochemicals are a requirement for the study of chemical interactions between plants. This paper describes a method for sample preparation and quantitative analysis of the allelopathic chemical content of rye ( Secale cereale L.) using g...

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Bibliographic Details
Published in:Journal of Chromatography A 2005-02, Vol.1066 (1), p.249-253
Main Authors: Finney, M.M., Danehower, D.A., Burton, J.D.
Format: Article
Language:English
Subjects:
3-Hydroxybutyric Acid - analysis
Agronomy. Soil science and plant productions
Allelochemicals
Allelopathy
Benzoxazines
Benzoxazinones
Benzoxazoles - analysis
Biological and medical sciences
BOA
Chemical constitution
Chromatography, Gas - methods
DIBOA
DIBOA-glucoside
Economic plant physiology
Food industries
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
General aspects
Glucosides - analysis
Herbicides - analysis
Hydroxamic acids
Hydroxybutyric acid
Lactates - analysis
Methods of analysis, processing and quality control, regulation, standards
Oxazines - analysis
Phenyllactic acid
Rye
Secale - chemistry
Secale cereale
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Summary:Accurate and reproducible methods for the analysis of plant allelochemicals are a requirement for the study of chemical interactions between plants. This paper describes a method for sample preparation and quantitative analysis of the allelopathic chemical content of rye ( Secale cereale L.) using gas chromatography (GC). Sample preparation consists of extraction of freeze-dried rye vegetative tissue with aqueous ethanol followed by partitioning of the allelochemicals into ethyl acetate, evaporation, and derivatization using the trimethylsilylating reagent N-methyl- N-trimethylsilyltrifluoroacetamide. GC analysis of the silylated mixture was performed using flame ionization detection. This method permits analysis of all known rye allelopathic agents including 2,4-dihydroxy-1,4-benzoxazin-3-one, its corresponding glucoside, 2-benzoxazolinone, β-hydroxybutyric acid, and β-phenyllactic acid. Identities of all compounds were confirmed by GC/MS analysis.
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.01.050