Simultaneous analysis of antioxidants and preservatives in cosmetics by supercritical fluid extraction combined with liquid chromatography–mass spectrometry

• Published in: Journal of Chromatography A 2006-07, Vol.1120 (1), p.244-251
• Main Authors: Lee, Maw-Rong, Lin, Chueh-Yu, Li, Zu-Guang, Tsai, Tzu-Feng
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Antioxidants; Antioxidants - analysis; Antioxidants - chemistry; Antioxidants - isolation & purification; Applied sciences; Butylated Hydroxyanisole - analysis; Butylated Hydroxyanisole - chemistry; Butylated Hydroxyanisole - isolation & purification; Butylated Hydroxytoluene - analysis; Butylated Hydroxytoluene - chemistry; Butylated Hydroxytoluene - isolation & purification; Chemical industry and chemicals; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Supercritical Fluid - methods; Cosmetics; Cosmetics - analysis; Cosmetics - chemistry; Cosmetics, toiletries; Exact sciences and technology; Mass spectrometry; Molecular Structure; Other chromatographic methods; Parabens - analysis; Parabens - chemistry; Parabens - isolation & purification; Preservatives; Preservatives, Pharmaceutical - analysis; Preservatives, Pharmaceutical - chemistry; Preservatives, Pharmaceutical - isolation & purification; Pressure; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization - methods; Supercritical fluid extraction; Temperature; Washing products. Cosmetics and toiletries. Perfumes
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2006.01.075

This study evaluated supercritical fluid extraction (SFE) combined with liquid chromatography–mass spectrometry (LC–MS) to determine trace preservatives and antioxidants including methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol (α-t) and α-tocopherol acetate (α-ta) in cosmetic products. A supercritical fluid extraction procedure was used to isolate four paraben preservatives and four antioxidants from the cosmetic matrix before quantitative analysis. The optimum extraction condition was performed with static extraction for 5 min, then dynamic extraction for 20 min by using carbon dioxide supercritical fluid at 14,000 kPa and 65 °C. Methanol was used as collection solvent and the sea sand was chosen as a filling material. The analytes were separated on a C18 reversed-phase column using methanol–water as mobile phase and quantified by measuring its mass spectrometry. The linearity range is from 10 to 20,000 ng/g with RSD values below 18%. Detection limits are achieved at the level of 4.7–142 ng/g. It was successfully applied to the determination of paraben preservatives and antioxidants in cosmetics without tedious pretreatment.