Determination of Thiamethoxam residues in honeybees by high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor

A method for the determination of Thiamethoxam in bee samples was set up by means of high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor (HPLC–hν–ED). Analytical method was based on a rapid sample extraction procedure with acetone, followed b...

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Bibliographic Details
Published in:Journal of Chromatography A 2006-08, Vol.1123 (1), p.60-65
Main Authors: Rancan, Milena, Rossi, Simona, Sabatini, Anna Gloria
Format: Article
Language:English
Subjects:
Animal productions
Apiculture
Biological and medical sciences
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Electrochemical detector
Electrochemistry - instrumentation
Fundamental and applied biological sciences. Psychology
Honeybee
HPLC
Insecta
Invertebrates
Neonicotinoid
Neonicotinoids
Nitro Compounds - analysis
Oxazines - analysis
Pesticide
Pesticide Residues - analysis
Photoactivation
Photochemistry - instrumentation
Sensitivity and Specificity
Terrestrial animal productions
Thiamethoxam
Thiazoles
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Summary:A method for the determination of Thiamethoxam in bee samples was set up by means of high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor (HPLC–hν–ED). Analytical method was based on a rapid sample extraction procedure with acetone, followed by chromatographic separation into a C18-RP column isocratically operated by 60 mM phosphate buffer/acetonitrile (75/25) mobile phase at pH 2.7. A photochemical reactor was used as a tool to verify and eventually quantify the presence of Thiamethoxam in the samples by distinguishing it from interference contribution. Detection was performed with a potential of 880 mV after a photoactivation with a 254 nm light. The least detectable dose was 0.002 mg kg −1. Recovery rates ranged between 59.88 and 71.62%.
ISSN:0021-9673
DOI:10.1016/j.chroma.2006.05.006