Solving matrix effects exploiting the second-order advantage in the resolution and determination of eight tetracycline antibiotics in effluent wastewater by modelling liquid chromatography data with multivariate curve resolution-alternating least squares and unfolded-partial least squares followed by residual bilinearization algorithms

A new powerful algorithm (unfolded-partial least squares followed by residual bilinearization (U-PLS/RBL)) was applied for first time on second-order liquid chromatography with diode array detection (LC–DAD) data and compared with a well-known established method (multivariate curve resolution-altern...

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Bibliographic Details
Published in:Journal of Chromatography A 2008, Vol.1179 (2), p.115-124
Main Authors: García, M.D. Gil, Culzoni, M.J., De Zan, M.M., Valverde, R. Santiago, Galera, M. Martínez, Goicoechea, H.C.
Format: Article
Language:English
Subjects:
algorithms
Antibiotics
chlortetracycline
demeclocycline
doxycycline
effluents
least squares
liquid chromatography
minocycline
oxytetracycline
pretreatment
Second-order advantage
tetracycline
Wastewater
water analysis
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Summary:A new powerful algorithm (unfolded-partial least squares followed by residual bilinearization (U-PLS/RBL)) was applied for first time on second-order liquid chromatography with diode array detection (LC–DAD) data and compared with a well-known established method (multivariate curve resolution-alternating least squares (MCR-ALS)) for the simultaneous determination of eight tetracyclines (tetracycline, oxytetracycline, meclocycline, minocycline, metacycline, chlortetracycline, demeclocycline and doxycycline) in wastewaters. Tetracyclines were pre-concentrated using Oasis Max C 18 cartridges and then separated on a Thermo Aquasil C18 (150 mm × 4.6 mm, 5 μm) column. The whole method was validated using Milli-Q water samples and both univariate and multivariate analytical figures of merit were obtained. Additionally, two data pre-treatment were applied (baseline correction and piecewise direct standardization), which allowed to correct the effect of breakthrough and to reduce the total interferences retained after pre-concentration of wastewaters. The results showed that the eight tetracycline antibiotics can be successfully determined in wastewaters, the drawbacks due to matrix interferences being adequately handled and overcome by using U-PSL/RBL.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.11.049