Simple preparation of baicalin from Scutellariae Radix

A simple, rapid and reproducible method was developed to isolate high-purity baicalin from Scutellariae Radix, the dried roots of Scutellaria baicalensis Georgi. The method involves partition/recrystallization steps without repeated column chromatography or special instruments. Isolated baicalin was...

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Bibliographic Details
Published in:Journal of Chromatography A 2009-03, Vol.1216 (11), p.2192-2194
Main Authors: Ohkoshi, Emika, Nagashima, Tomomi, Sato, Hiroyasu, Fujii, Yuichi, Nozawa, Koohei, Nagai, Masahiro
Format: Article
Language:English
Subjects:
Baicalein
Baicalin
flavones
herbal medicines
Oriental traditional medicine
Preparation
Purification
roots
Scutellaria baicalensis
Scutellariae Radix
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Summary:A simple, rapid and reproducible method was developed to isolate high-purity baicalin from Scutellariae Radix, the dried roots of Scutellaria baicalensis Georgi. The method involves partition/recrystallization steps without repeated column chromatography or special instruments. Isolated baicalin was characterized by comparisons of TLC, HPLC, IR, MS, and NMR data with an authentic sample. Moreover, β-glucuronidase hydrolysis of baicalin sequentially yielded glucuronic acid and baicalein as confirmed by co-TLC with authentic samples. The purity of baicalin was more than 97% with yield ca. 8.7% (w/w). The method presented here appears suitable for commercial application.
ISSN:0021-9673
DOI:10.1016/j.chroma.2008.03.059