Two-dimensional turbulent flow chromatography coupled on-line to liquid chromatography–mass spectrometry for solution-based ligand screening against multiple proteins

• Published in: Journal of Chromatography A 2009-03, Vol.1216 (12), p.2394-2403
• Main Authors: Zhou, Jian-Liang, An, Jing-Jing, Li, Ping, Li, Hui-Jun, Jiang, Yan, Cheng, Jie-Fei
• Format: Article
• Language: English
• Subjects: AChE; Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; BChE; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Ligand screening; On-line bioseparation/chemical analysis strategy; Turbulent flow chromatography; Two-dimensional
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2009.01.010

We present herein a novel bioseparation/chemical analysis strategy for protein–ligand screening and affinity ranking in compound mixtures, designed to increase screening rates and improve sensitivity and ruggedness in performance. The strategy is carried out by combining on-line two-dimensional turbulent flow chromatography (2D-TFC) with liquid chromatography–mass spectrometry (LC–MS), and accomplished through the following steps: (1) a reversed-phase TFC stage to separate the protein/ligand complex from the unbound free molecules, (2) an on-line dissociation process to release the bound ligands from the complexes, and (3) a second mixed-mode cation-exchange/reversed-phase TFC stage to trap the bound ligands and to remove the proteins and salts, followed by LC–MS analysis for identification and determination of the binding affinities. The technique can implement an ultra-fast isolation of protein/ligand complex with the retention time of a complex peak in about 5 s, and on-line prepare the “clean” sample to be directly compatible with the LC–MS analysis. The improvement in performance of this 2D-TFC/LC–MS approach over the conventional approach has been demonstrated by determining affinity-selected ligands of the target proteins acetylcholinesterase and butyrylcholinesterase from a small library with known binding affinities and a steroidal alkaloid library composed of structurally similar compounds. Our results show that 2D-TFC/LC–MS is a generic and efficient tool for high-throughput screening of ligands with low-to-high binding affinities, and structure-activity relationship evaluation.