Investigation of porous graphitic carbon for triterpenoids and natural resinous materials analysis by high performance liquid chromatography hyphenated to mass spectrometry

► Separation of triterpenes in resinous materials on PGC–MS is optimised. ► Varying weak solvents have greater effect on porous graphitic carbon retention. ► Electronic modifier in the mobile phase has no influence on triterpenes retention. Natural resinous materials are mainly composed of pentacycl...

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Bibliographic Details
Published in:Journal of Chromatography A 2012-06, Vol.1240, p.140-146
Main Authors: Rhourri-Frih, B., Chaimbault, P., Dequeral, D., André, P., Lafosse, M.
Format: Article
Language:English
Subjects:
active ingredients
atmospheric pressure
carbon
formic acid
high performance liquid chromatography
HPLC–APPI-MS
isomers
Isomers separation
mass spectrometry
Pentacyclic triterpenes
Porous graphitic carbon
Resinous materials
screening
temperature
triterpenoids
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Summary:► Separation of triterpenes in resinous materials on PGC–MS is optimised. ► Varying weak solvents have greater effect on porous graphitic carbon retention. ► Electronic modifier in the mobile phase has no influence on triterpenes retention. Natural resinous materials are mainly composed of pentacyclic triterpenes which exhibit a large number of interesting medicinal activities. However, the presence of numerous isomers within the active substances makes their screening by HPLC very challenging. Porous graphitic carbon was investigated as stationary phase to achieve triterpenes isomers separation. The influence of various parameters (temperature, formic acid concentration and mobile phase composition) on the retention was considered. A usual decrease of the retention of triterpenes was observed with the increase of the temperature. Therefore, separation in resinous materials was performed at 25°C. Acetonitrile–isopropanol mixture was chosen as mobile phase in gradient elution and leads to the best compromise between efficiency and high resolution. The lack of chromophore groups in the pentacyclic triterpenes structures required the use of mass spectrometry detection. Moreover, atmospheric pressure photo-ionisation mass spectrometry prevents compounds fragmentation which was helpful for spectra interpretation and compounds identification.
ISSN:0021-9673
DOI:10.1016/j.chroma.2012.03.094