High-accuracy analysis system for the redox status of coenzyme Q10 by online supercritical fluid extraction–supercritical fluid chromatography/mass spectrometry

► Analysis of CoQ10 by online supercritical fluid extraction (SFE)–SFC–MS. ► Only 0.05mg of dried photosynthesis bacterium was needed for the analysis. ► A higher ratio of the reduced form of CoQ10 was detected by online SFE. ► Highly accurate analysis of easily oxidized compounds can be performed....

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2012-08, Vol.1250, p.76-79
Main Authors: Matsubara, Atsuki, Harada, Kazuo, Hirata, Kazumasa, Fukusaki, Eiichiro, Bamba, Takeshi
Format: Article
Language:English
Subjects:
chromatography
Coenzyme Q10
Mass spectrometry
Online extraction
oxidation
oxidative stress
solvents
Supercritical fluid chromatography
Supercritical fluid extraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► Analysis of CoQ10 by online supercritical fluid extraction (SFE)–SFC–MS. ► Only 0.05mg of dried photosynthesis bacterium was needed for the analysis. ► A higher ratio of the reduced form of CoQ10 was detected by online SFE. ► Highly accurate analysis of easily oxidized compounds can be performed. The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction–supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q10 (CoQ10) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.
ISSN:0021-9673
DOI:10.1016/j.chroma.2012.05.009