Analysis of fatty acid samples by hydrophilic interaction liquid chromatography and charged aerosol detector

• Published in: Journal of Chromatography A 2015-02, Vol.1383, p.121-126
• Main Authors: Roy, Claude-Eric, Kauss, Tina, Prevot, Sandrine, Barthelemy, Philippe, Gaudin, Karen
• Format: Article
• Language: English
• Subjects: CAD; Fatty acid; HILIC; PGI2; Prostacyclin; Prostaglandin
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2015.01.046

•First HILIC method for PGI2 analysis.•The separation of PGI2 with its main degradation product was performed in 1.60min.•Fatty acids and PGI2 derivatives were analyzed by HILIC hyphenated to CAD.•PGI2 derivatives had similar response with CAD.•Green solvent such acetone was candidate for elution of lipids in HILIC/CAD approach. HILIC/CAD techniques were used in analysis of samples containing fatty acids. Amine base column appeared to be the more retentive stationary phase compared to zwitterionic and BEH silica. The retention decreased with pH mobile phases changing from 3 to 5. Acetonitrile and acetone organic modifier were compared. Acetone gave higher eluotropic strength and better peak symmetry whereas acetonitrile led to higher efficiency. The retention decreased when ammonium acetate concentration increased from 5 to 20mM. The use of sub-2μm column did not show flat Van Deemter curves at high flow rates. A rapid separation of PGI2 and its main degradation product, 6-keto prostaglandin F1α was obtained in 1.6min with a Hypersil GOLD, 50mm×2.1mm, 1.9μm with; acetonitrile/acetate ammonium pH 5 at 20mM (85/15; v/v at 0.7ml/min).