Size fractionation and size characterization of nanoemulsions of lipid droplets and large unilamellar lipid vesicles by asymmetric-flow field-flow fractionation/multi-angle light scattering and dynamic light scattering

•Lipid droplets and lipid vesicles of different chemical composition were prepared.•Size characteristics of lipid particles were determined by AF4-MALS, DLS and TEM.•Shape and internal structure of lipid particles was inferred from the shape factor.•Size dispersity of lipid vesicles containing highe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2015-10, Vol.1418, p.185-191
Main Authors: Vezočnik, Valerija, Rebolj, Katja, Sitar, Simona, Ota, Katja, Tušek-Žnidarič, Magda, Štrus, Jasna, Sepčić, Kristina, Pahovnik, David, Maček, Peter, Žagar, Ema
Format: Article
Language:English
Subjects:
Cholesterol
Field-flow fractionation
Lipid droplets
Lipid vesicles
Particle size
Sphingomyelin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Lipid droplets and lipid vesicles of different chemical composition were prepared.•Size characteristics of lipid particles were determined by AF4-MALS, DLS and TEM.•Shape and internal structure of lipid particles was inferred from the shape factor.•Size dispersity of lipid vesicles containing higher cholesterol amount was broader.•Lipid vesicles showed narrower size distribution than lipid droplets. Asymmetric-flow field-flow fractionation technique coupled to a multi-angle light-scattering detector (AF4-MALS) was used together with dynamic light-scattering (DLS) in batch mode and transmission electron microscopy (TEM) to study the size characteristics of the trioleoylglycerol lipid droplets covered by a monolayer of sphingomyelin and cholesterol, in water phase. These lipid droplet nanoemulsions (LD) were formed by ultrasonication. In parallel, the size characteristics of large unilamellar lipid vesicles (LUV) prepared by extrusion and composed of sphingomyelin and cholesterol were determined. LD and LUV were prepared at two different molar ratios (1/1, 4/1) of sphingomyelin and cholesterol. In AF4-MALS, various cross-flow conditions and mobile phase compositions were tested to optimize the separation of LD or LUV particles. The particle radii, R, as well as the root-mean-square radii, Rrms, of LD and LUV were determined by AF4-MALS, whereas the hydrodynamic radii, Rh, were obtained by DLS. TEM visualization revealed round shape particles of LD and LUV.
ISSN:0021-9673
DOI:10.1016/j.chroma.2015.09.048