Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy

• Published in: Journal of Chromatography A 2017-02, Vol.1485, p.142-146
• Main Authors: Chong, Kah Chun, Thang, Lee Yien, Quirino, Joselito P., See, Hong Heng
• Format: Article
• Language: English
• Subjects: Contactless conductivity detection; Field-enhance sample injection; Micelle-to-solvent stacking; Microchip electrophoresis; Vancomycin
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2017.01.012

•Multi-stacking technique based on FESI and MSS was developed in aqueous-based MCE-C4D.•New approach was evaluated for the monitoring of vancomycin in human plasma.•LOD of 1.2μg/mL and SEF of 83-fold compared to typical gated injection method were obtained. A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C4D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in MCE-C4D system equipped with a commercially available double T-junction glass chip. The cationic analytes from the two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample-loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte and a sensitivity enhancement factor (SEF) of up to 217 was achieved. The developed approach was subsequently implemented in the aqueous-based MCE, coupled to C4D in order to monitor the targeted antibiotic (in this case, vancomycin) present in human plasma samples. The multi-stacking and analysis time for vancomycin were 50s and 250s respectively, with SEF of approximately 83 when compared to typical gated injection. The detection limit of the method for vancomycin was 1.2μg/mL, with intraday and interday repeatability RSDs of 2.6% and 4.3%, respectively. Recoveries in spiked human plasma were 99.0%–99.2%.