Magnetic porous β-cyclodextrin polymer for magnetic solid-phase extraction of microcystins from environmental water samples

• Published in: Journal of Chromatography A 2017-06, Vol.1503, p.1-11
• Main Authors: Zhang, Wenmin, Lin, Mingxia, Wang, Meili, Tong, Ping, Lu, Qiaomei, Zhang, Lan
• Format: Article
• Language: English
• Subjects: HPLC–MS/MS; Magnetic solid-phase extraction; Microcystins; Porous β-cyclodextrin polymer
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2017.04.063

•Magnetization of P-CDP was creatively prepared as a MSPE sorbent.•Magnetic P-CDP showed excellent extraction performance for MCs, especially for MC-RR.•The developed method was free from interference of common ions in water samples.•The developed MSPE-HPLC–MS/MS method was very simple, efficient and sensitive. Microcystins (MCs) are cyclic heptapeptide toxins and tumor promoters produced by cyanobacteria, which threaten the health of humans. In this study, magnetic porous β-cyclodextrin polymer (Fe3O4@SiO2@P-CDP) was synthesized and characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray spectrometry, Fourier transform infrared spectrometry, X-ray diffraction, nitrogen adsorption porosimetry and vibrating sample magnetometer. The synthesized Fe3O4@SiO2@P-CDP particles were then used for magnetic solid-phase extraction (MSPE) of MCs from environmental water samples, and exhibited excellent extraction performance, especially for MC-RR. Coupled with high-performance liquid chromatography with tandem mass spectrometry (HPLC–MS/MS), a simple, efficient and sensitive method for determination of trace levels of MCs was established. After the optimization of conditions, wide linear ranges (2.0–1000pgmL−1), good linearity (r2≥0.9996) and acceptable repeatability (RSD≤9.4%, n=5) were obtained. The limits of detection (LODs, S/N=3) and limits of quantification (LOQs, S/N=10) for three MCs (MC-LR, MC-RR and MC-YR) were in the range of 1.0–2.0pgmL−1 and 2.0–5.0pgmL−1, respectively. Typical water samples were analyzed by the developed method, and trace levels of MC-LR and MC-RR were detected. The results demonstrate that the developed method has great potential for the determination of MCs in complicated matrix.