Loading…
Effect directed analysis and TLC screening of Schisandra chinensis fruits
•Effect directed analysis and TLC screening of S. chinensis fruits were performed.•Inhibition of acetylcholinesterase related to lignans was confirmed.•TLC-DB showed activity against Bacillus subtilis.•Citric acid is probably responsible for antibacterial properties of S. chinensis.•Bioactive compon...
Saved in:
Published in: | Journal of Chromatography A 2020-05, Vol.1618, p.460942, Article 460942 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | •Effect directed analysis and TLC screening of S. chinensis fruits were performed.•Inhibition of acetylcholinesterase related to lignans was confirmed.•TLC-DB showed activity against Bacillus subtilis.•Citric acid is probably responsible for antibacterial properties of S. chinensis.•Bioactive components were tentatively identified by ESI-LC-MS.
The fruit of Schisandra chinensis (Chinese magnolia vine), the medicinal plant well-known in Traditional Chinese Medicine, gains great popularity in the modern phytopharmacology. This phenomena is related to the wide and powerful healing properties, including supporting immune, nervous and digestive systems activity. S. chinensis is also known for its adaptogenic properties which can support the treatment of neurodegenerative disorders, particularly Alzheimer's disease. The components of S. chinensis have been analyzed mostly using chromatography, including HPLC, GC and TLC. The last technique can be easily hyphenated with biological assays performed directly on a TLC plate that is using effect directed detection.
TLC-direct bioautography (TLC-DB) for acetylcholinesterase (AChE) inhibition and TLC-DB against Bacillus subtilis were performed, followed by micro-preparative separation of fractions which were subsequently subjected to LC-MS tentative identification. Additionally, screening analysis was done using both biological detection and derivatization reagents (e.g. PMA, thymol, NP-PEG). Both TLC screening and effect-directed analysis (TLC-DB followed by MS analysis) of S. chinensis fruit revealed components with biological activity, especially antibacterials (e.g. citric acid) and inhibitors of AChE (mainly dibenzocyclooctadiene lignans).
AChE inhibition activities were confirmed by TLC-DB for nine standards that is: 6-O-benzoylgomisin, deoxyschisandrin, gomisin A, gomisin G, schisandrin, schisandrin C, schisanhenol, schisantherin A and schisantherin B. These lignans were further identified by LC-ESI-MS in the isolated fraction revealing AChE inhibition. Moreover three other lignans: γ-schisandrin, schisandrin B and gomisin J were tentatively identified by LC-ESI-MS. |
---|---|
ISSN: | 0021-9673 |
DOI: | 10.1016/j.chroma.2020.460942 |