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Analysis of 2-aminopyridine labeled glycans by dual-mode online solid phase extraction for hydrophilic interaction and reversed-phase liquid chromatography

•A simple procedure was developed to remove extra 2-aminopyridine using on-line SPE.•A cation exchange column was used to separate labeled glycans from excess reagents.•An ODS mini column with a 6-port valve was used to trap labeled glycans.•The method was applicable to HILIC and RP separation of gl...

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Bibliographic Details
Published in:Journal of Chromatography A 2020-08, Vol.1625, p.461194, Article 461194
Main Authors: Kishimoto, Yuka, Okada, Fuka, Maesako, Tomohiro, Yamamoto, Sachio, Kinoshita, Mitsuhiro, Hayakawa, Takao, Suzuki, Shigeo
Format: Article
Language:English
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Summary:•A simple procedure was developed to remove extra 2-aminopyridine using on-line SPE.•A cation exchange column was used to separate labeled glycans from excess reagents.•An ODS mini column with a 6-port valve was used to trap labeled glycans.•The method was applicable to HILIC and RP separation of glycan derivatives. Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity. However, the methods required to remove large amounts of excess labeling reagents from the reaction mixture are time-consuming. Furthermore, these methods, including solvent extraction and solid phase extraction (SPE), often impair quantitative analysis. Here, we developed an online sample cleanup procedure for HPLC analysis of 2-aminopyridine (AP)-labeled glycans using a six-port/two-way valve and two small columns: one packed with a strong cation exchange resin (SCX) and the other comprising ODS silica gel. AP-labeled glycans delivered from an injection port were separated from excess AP by passing through an SCX column (4.6 mm i.d., 1 cm long) regulated to 40°C. The AP-labeled glycans were trapped on an ODS column (4.6 mm i.d., 1 cm long) to further separate them from inorganic contaminants. By changing the valve position after 2 min to connect the ODS column to an analysis column, AP-labeled glycans trapped in the ODS column were eluted with an acetonitrile-containing eluent followed by hydrophilic interaction liquid chromatography (HILIC) separation on an amide column or reversed-phase mode separation on a C30 column. This method was successfully used to analyze N-linked glycans released from several glycoprotein samples.
ISSN:0021-9673
DOI:10.1016/j.chroma.2020.461194