Development of clinoptilolite zeolite-coated magnetic nanocomposite-based solid phase microextraction method for the determination of Rhodamine B in cosmetic products

• Published in: Journal of Chromatography A 2022-09, Vol.1680, p.463433, Article 463433
• Main Authors: Dalmaz, Aslihan, Sivrikaya Özak, Sezen
• Format: Article
• Language: English
• Subjects: Clinoptilolite zeolite/Fe3O4 nanocomposite; Cosmetic product; Green synthesis; Laurus Nobilis L; Rhodamine B
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2022.463433

•Rhodamine B extraction was carried out by combining the Zeolite@Fe3O4-MSPE method.•The sensitivity of the HPLC-UV system has been improved 40 times.•High recovery values in the range of 92-101% were obtained in cosmetic samples.•A low LOD value was obtained for rhodamine B at 0.16 ng mL−1. Green synthesis of clinoptilolite zeolite/Fe3O4 nanocomposite (MZNC) was carried out using Laurus Nobilis L. leaf extract. Characterization of this MZNC was performed using Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, X-Ray Diffraction and Vibrating Sample Magnetometer. According to the VSM analysis results, the saturation magnetization of 23 emu/g and coercivity of 23.5 Oe indicate that the synthesized magnetic nanocomposite is superparamagnetic. A new ultrasonic assisted clinoptilolite zeolite-coated magnetic nanocomposite-based solid phase microextraction (MZNC-SPME) method combined with high performance liquid chromatography was developed for the extraction and determination of Rhodamine B. The preconcentration factor for the MZNC-SPME method was found to be 40 under optimal conditions. Under optimal conditions, the linear range, correlation coefficient (R2), limit of detection (LOD), and intra- and interday relative standard deviation (RSD) were found to be 1.00–100.00 ng mL−1, 0.9995, 0.16 ng mL−1, 1.89% and 2.49%, respectively. The developed method was successfully performed to determine Rhodamine B in 6 different cosmetic samples. 6 ions and 5 different dyes were added to the sample solution to show the selectivity of the method. The obtained results show that the determination of Rhodamine B is possible in the presence of these ions and dyes. In order to determine the accuracy of the MZNC-SPME method, two different concentrations of Rhodamine B concentration in cosmetic samples were added as 10 and 50 ng mL−1. Extraction recoveries were found in the range of 92.03-101.52% and these results are quite satisfactory. It is seen that the developed method for the extraction and determination of Rhodamine B from cosmetic samples is applicable due to the easy synthesis of the sorbent and the short, simple, environmentally friendly and low cost of the method.