Arsenosugar extracted from algae: Isolation by anionic exchange solid‐phase extraction

• Published in: Journal of Chromatography A 2022-11, Vol.1684, p.463549, Article 463549
• Main Authors: Morales-Rodríguez, Alba, Pérez-López, Miquel, Puigpelat, Elle, Sahuquillo, Àngels, Barrón, Dolores, López-Sánchez, José Fermín
• Format: Article
• Language: English
• Subjects: Algae; Arsenosugars; IC-ICP-MS; strong anion exchange-SPE
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2022.463549

•The behavior of arsenosugars on anion exchange-SPE is study for first time.•SPE was assessed as a clean-up method of arsenosugar from algae water extract.•SPE allows to isolate arsenosugars as the only arsenic species present in the solution. Obtaining reliable speciation data for evaluating dietary exposure, and increasing understanding of arsenic biochemistry in algae, are hindered by the availability of suitable standards of arsenosugars, the major species in these types of samples. Moreover, chemical syntheses of such compounds have been reported to be complex and tedious. The aim of this work was to investigate the feasibility of the anionic exchange SPE cartridges (SAX and WAX) as an easy and quick alternative for the isolation and preconcentration of arsenosugars. Two commercial silica-based SPE cartridges strong anion exchange sorbent (DSC-SAX) and weak anion exchange sorbent (DSC-NH2) were compared for the SPE of three arsenosugars (PO4-Sug, SO3-Sug and SO4-Sug). The effect of pH, ionic strength, type of salt and elution solvent on the elution protocols of these arsenosugars are studied. Eluted solutions from SPE were analyzed by ICP-MS for total arsenic content and IC-ICP-MS for the study of arsenic speciation. The developed SPE procedure allows to obtain a solution containing the three arsenosugars isolated from other arsenic species with recoveries over 75% for SO3-Sug and SO4-Sug, whereas for PO4-Sug were around 45%.